Recombinant Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (ab248786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free
See all 14-3-3 gamma/YWHAG primary antibodies -
Description
Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, A431 and HeLa cell lysates.
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General notes
ab248786 is the carrier-free version of ab137048.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9654(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsPhosphorylated by various PKC isozymes. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Omim: 605356 Human
- SwissProt: P61981 Human
- SwissProt: P61982 Mouse
- SwissProt: P61983 Rat
- Unigene: 721582 Human
see all -
Alternative names
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Images
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : K562 lysate
Lane 3 : A549 lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab137048, the same antibody clone in a different buffer formulation.
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : YWHAG knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab137048).
Lanes 1-4: Merged signal (red and green). Green - ab137048 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab137048 Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248786 has not yet been referenced specifically in any publications.