Anti-14-3-3 (phospho S58) antibody (ab30554)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
  • Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (ab30554)
  • Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)

Key features and details

  • Rabbit polyclonal to 14-3-3 (phospho S58)
  • Suitable for: WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human, Zebrafish
  • Isotype: IgG

Overview

  • Product name

    Anti-14-3-3 (phospho S58) antibody
    See all 14-3-3 primary antibodies

  • Description

    Rabbit polyclonal to 14-3-3 (phospho S58)

  • Host species

    Rabbit

  • Specificity

    Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

    The N-terminal sequence used as antigen has high homology across a wide variety of species for all of the 14-3-3 proteins. We have not specifically tested it for reactivity against each protein in any given species, we do expect it to recognize most all of them.

  • Tested applications

    Suitable for: WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Sheep, Chicken, Cow, Dog, Xenopus laevis, Non human primates

  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab30554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa.
IHC-Fr 1/500.

Target

Images

  • Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
    Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
    All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution

    Lane 1 : Rat brain stem lysate
    Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen

    Observed band size: 29 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (ab30554)
    Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (ab30554)
    ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.
  • Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
    Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
    All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate (20ug)
    Lane 3 : Mouse brain homogenate (20ug)

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

References (2)

Publishing research using ab30554? Please let us know so that we can cite the reference in this datasheet.

ab30554 has been referenced in 2 publications.

  • Civiero L  et al. PAK6 Phosphorylates 14-3-3? to Regulate Steady State Phosphorylation of LRRK2. Front Mol Neurosci 10:417 (2017). PubMed: 29311810
  • Okada N  et al. A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. J Cell Sci 124:57-67 (2011). WB, ICC/IF ; Human . PubMed: 21118956

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Immunohistochemistry (Frozen sections) abreview for Anti-14-3-3 (phospho S58) antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample
Mouse Cell (Mouse colon.)
Specification
Mouse colon.
Permeabilization
No
Fixative
PFA/Ethanol
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Verified customer

Submitted Aug 01 2014

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