Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 sigma/SFN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 154-247.
    Database link: P31947

  • Positive control

    • 293T, A431, HeLa, HepG2, Neuro2A, GL261 and PC12 whole cell lysates; A431 cells; Human 59T xenograft tissue.
  • General notes

     This product was previously labelled as 14-3-3 sigma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab151504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity

    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Images

  • All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 27 kDa



    12% SDS PAGE
  • All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma/SFN with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.

  • Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma/SFN with ab151504 at 1/100 dilution.

References

ab151504 has not yet been referenced specifically in any publications.

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