Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Goat polyclonal to 14-3-3 sigma/SFN
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow trout
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN (internal sequence).
    Sequence:

    DLHTLSEDSYKDST


    Database link: P31947

  • Positive control

    • WB: Human, mouse and rat skin tissue lysates and A431 and U2OS cell lysates. IHC-P: Human skin tissue.
  • General notes

     This product was previously labelled as 14-3-3 sigma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 25298405

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity

    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Images

  • Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml + Human skin tissue lysate in RIPA buffer at 35 µg

    Predicted band size: 28 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml

    Lane 1 : A431 cell lysate in RIPA buffer
    Lane 2 : U2OS cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 28 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

  • All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml

    Lane 1 : Mouse skin tissue lysate in RIPA buffer
    Lane 2 : Rat skin tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 28 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

References

This product has been referenced in:

  • Plak K  et al. Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Mol Biol Cell 25:4195-204 (2014). ICC/IF ; Dictyostelium discoideum . Read more (PubMed: 25298405) »
See 1 Publication for this product

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