Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 sigma/SFN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 198-248.
    Database link: NP_006133.1

  • Positive control

  • General notes

     This product was previously labelled as 14-3-3 sigma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab86380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity

    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Images

  • All lanes : Anti-14-3-3 sigma/SFN antibody (ab86380) at 0.1 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma/SFN with ab86380 at 1/1000 (1µg/ml). Detection: DAB.

  • Detection of 14-3-3 sigma/SFN by Western blot of Immunprecipitate.
    ab86380, at 1 µg/ml, staining 14-3-3 sigma/SFN in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab86380 has not yet been referenced specifically in any publications.

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