Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody (Biotin)
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Goat polyclonal to 14-3-3 sigma/SFN (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Sheep, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 204-217 (internal sequence) (Cysteine residue). (NP_006133.1).
    Sequence:

    C-DLHTLSEDSYKDST


    Database link: P31947

  • Positive control

    • WB: Mouse skin lysate.
  • General notes

     This product was previously labelled as 14-3-3 sigma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab240484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 27 kDa.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity

    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Images

  • Anti-14-3-3 sigma/SFN antibody (Biotin) (ab240484) at 0.1 µg/ml + Mouse skin lysate in RIPA buffer at 35 µg

    Predicted band size: 27 kDa



    Primary incubation for 1 hour.

    Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

    The WB exactly mirrors the result obtained with the parental, non-biotinylated version of the antibody.

     

     

References

ab240484 has not yet been referenced specifically in any publications.

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