RecombinantRabMAb

Recombinant Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

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Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
  • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
  • Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]
    See all 14-3-3 sigma/SFN primary antibodies

  • Description

    Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human 14-3-3 sigma/SFN aa 50-150. The exact sequence is proprietary.
    Database link: P31947

  • Positive control

    • Human skin, A431 and HACAT lysates; Human 14-3-3 Sigma/SFN recombinant protein; Human tonsil tissue.

  • General notes

     

     

     This product was previously labelled as 14-3-3 sigma

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab193667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.

  • Tissue specificity

    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

  • Sequence similarities

    Belongs to the 14-3-3 family.

  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.

  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt

  • Database links

    • Alternative names

      • 14 3 3 protein sigma antibody
      • 14-3-3 protein sigma antibody
      • 1433S_HUMAN antibody
      • Epithelial cell marker protein 1 antibody
      • Er antibody
      • HME 1 antibody
      • HME1 antibody
      • MGC143283 antibody
      • Mkrn3 antibody
      • Mme1 antibody
      • OTTHUMP00000004242 antibody
      • RP23 137L22.11 antibody
      • SFN antibody
      • SFN protein antibody
      • Stratifin antibody
      • YWHAS antibody
      see all

    Images

    • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      All lanes : Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

      Lane 1 : Human skin lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HACAT cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Observed band size: 28 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?



      Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
      Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

    References (0)

    Publishing research using ab193667? Please let us know so that we can cite the reference in this datasheet.

    ab193667 has not yet been referenced specifically in any publications.

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