Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
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Overview
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Product nameAnti-14-3-3 sigma/SFN antibody [EPR5873(2)]
See all 14-3-3 sigma/SFN primary antibodies -
DescriptionRabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide within Human 14-3-3 sigma/SFN aa 50-150. The exact sequence is proprietary.
Database link: P31947 -
Positive control
- Human skin, A431 and HACAT lysates; Human 14-3-3 Sigma/SFN recombinant protein; Human tonsil tissue.
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General notes
This product was previously labelled as 14-3-3 sigma
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR5873(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab193667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 27 kDa). | |
| IHC-P | 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
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Sequence similaritiesBelongs to the 14-3-3 family.
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Post-translational
modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
- Information by UniProt
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Database links
- Entrez Gene: 2810 Human
- Entrez Gene: 313017 Rat
- Omim: 601290 Human
- SwissProt: P31947 Human
- Unigene: 523718 Human
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Alternative names
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
Images
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![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)](https://www.abcam.com/ps/products/193/ab193667/Images/ab193667-231098-ab193667WB.jpg)
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)All lanes : Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : A431 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)](https://www.abcam.com/ps/products/193/ab193667/Images/ab193667-231096-ab193667IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
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![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)](https://www.abcam.com/ps/products/193/ab193667/Images/ab193667-231097-ab193667WB2.jpg)
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab193667 has not yet been referenced specifically in any publications.
