Product nameAnti-14-3-3 tau antibody
See all 14-3-3 tau primary antibodies
DescriptionRabbit polyclonal to 14-3-3 tau
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Xenopus laevis, Orangutan
Synthetic peptide aa 195-245. The exact sequence is proprietary. NP_006817.1
Database link: P27348
- IHC-P: Human breast carcinoma tissue. WB: HeLa, HEK-239T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 28 kDa.|
RelevanceMembers of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions. the gene encoding 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
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All lanes : Anti-14-3-3 tau antibody (ab264319) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 30 seconds
14-3-3 tau was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264319 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264319 at 1 µg/ml.
Lane 1: ab264319 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling 14-3-3 tau with ab264319 at 1/5,000 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264319 has not yet been referenced specifically in any publications.