Key features and details
- Rabbit polyclonal to 14-3-3 Tau
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-14-3-3 Tau antibody
See all 14-3-3 Tau primary antibodies
DescriptionRabbit polyclonal to 14-3-3 Tau
SpecificityDetects endogenous levels of total 14-3-3 Tau protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human 14-3-3 Tau (internal sequence). The exact sequence is proprietary.
- WB: HUVEC and HeLa cell lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab64991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsSer-232 is probably phosphorylated by CK1.
Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.
- Information by UniProt
- 14 3 3 protein T cell antibody
- 14 3 3 protein tau antibody
- 14 3 3 protein theta antibody
All lanes : Anti-14-3-3 Tau antibody (ab64991) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells at 5-30ug with no immunizing peptide
Lane 2 : Extracts from HeLa cells at 5-30ug with no immunizing peptide
Lane 3 : Extracts from HeLa cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size: 28 kDa
Observed band size: 28 kDa
ab64991 has not yet been referenced specifically in any publications.