Key features and details
- Rabbit polyclonal to 14-3-3 Tau (phospho S232)
- Suitable for: ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-14-3-3 Tau (phospho S232) antibody
See all 14-3-3 Tau primary antibodies
DescriptionRabbit polyclonal to 14-3-3 Tau (phospho S232)
Specificityab63369 detects 14-3-3 Tau only when phosphorylated at serine 232.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human 14-3-3 Tau (phospho S232). (S-D-SP-A-G).
Database link: P27348
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsSer-232 is probably phosphorylated by CK1.
Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.
- Information by UniProt
- 14 3 3 protein T cell antibody
- 14 3 3 protein tau antibody
- 14 3 3 protein theta antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63369 has been referenced in 1 publication.
- McFerrin MB et al. Dysregulation of 14-3-3 proteins in neurodegenerative diseases with Lewy body or Alzheimer pathology. Ann Clin Transl Neurol 4:466-477 (2017). PubMed: 28695147