Product nameAnti-14-3-3 Theta + Tau antibody [EPR5123]
See all 14-3-3 Theta + Tau primary antibodies
DescriptionRabbit monoclonal [EPR5123] to 14-3-3 Theta + Tau
Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human 14-3-3 Theta + Tau (N terminal). The exact sequence is proprietary.
- HeLa, 293T, A549 and A431 cell lysates; A549 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.09 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|Flow Cyt||1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsSer-232 is probably phosphorylated by CK1.
Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.
- Information by UniProt
- 14-3-3 protein T cell antibody
- 14-3-3 protein T-cell antibody
- 14-3-3 protein tau antibody
All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
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