Key features and details
- Mouse monoclonal [1314CT422.214.171.124.140] to 14-3-3 zeta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Product nameAnti-14-3-3 zeta antibody [1314CT4126.96.36.199.140]
See all 14-3-3 zeta primary antibodies
DescriptionMouse monoclonal [1314CT4188.8.131.52.140] to 14-3-3 zeta
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human 14-3-3 zeta aa 1-245.
Database link: P63104
- A431, HepG2, NIH-3T3, PC-12, C6 and Mouse brain lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab188368 is purified through a protein G column, followed by dialysis against PBS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab188368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 28 kDa.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
Cellular localizationCytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
- Information by UniProt
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
All lanes : Anti-14-3-3 zeta antibody [1314CT4184.108.40.206.140] (ab188368) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : NIH-3T3 cell lysate
Lane 4 : PC-12 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-mouse IgG H&L(HRP) at 1/5000 dilution
Predicted band size: 28 kDa
ab188368 has not yet been referenced specifically in any publications.