Overview

  • Product name

  • Description

    Rabbit polyclonal to 14-3-3 zeta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 72-102 of Human 14-3-3 zeta.

  • Positive control

    • MDA-MB-231 and A2058 cell lysates; Mouse cerebellum and Mouse brain tissue lysates; Mouse brain tissue; MDA-MB-231 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab135823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localization

    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Images

  • All lanes : Anti-14-3-3 zeta antibody (ab135823) at 1/100 dilution

    Lane 1 : MDA-MB-231 cell lysate
    Lane 2 : A2058 cell lysate
    Lane 3 : Mouse cerebellum tissue lysate
    Lane 4 : Mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse brain tissue labelling 14-3-3 zeta with ab135823 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow Cytometric analysis of MDA-MB-231 cells labelling 14-3-3 zeta with ab135823 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135823 has not yet been referenced specifically in any publications.

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