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    14-3-3-zeta-antibody-ab51129.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-14-3-3 zeta antibody (ab51129)

  • Datasheet
Reviews (3) Submit a question References (20)

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Western blot - Anti-14-3-3 zeta antibody (ab51129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)
  • Western blot - Anti-14-3-3 zeta antibody (ab51129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)
  • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)

Key features and details

  • Rabbit polyclonal to 14-3-3 zeta
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human 14-3-3 zeta protein (ab87361)

View more associated products

Overview

  • Product name

    Anti-14-3-3 zeta antibody
    See all 14-3-3 zeta primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 zeta
  • Host species

    Rabbit
  • Specificity

    ab51129 detects endogenous levels of total 14-3-3 zeta protein.

    Cross reactivity of ab51129 antibody with other member of the 14-3-3 family:

    -92% identities with 14-3-3  beta/alpha, gamma and eta
    -77% identities with 14-3-3 teta
    -69% identities with 14-3-3 epsilon
    -62% identities with 14-3-3 sigma

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 zeta. The immunogen is a 13 amino acid synthetic peptide that resides within amino acids 24-73 of the human 14-3-3 zeta.
    Database link: P63104

  • Positive control

    • WB: 293 cells IHC-P: Human glioblastoma, human breast carcinoma tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human 14-3-3 zeta protein (ab87361)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (3)
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/50 - 1/100.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localization

    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Target information above from: UniProt accession P63104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7534 Human
    • Omim: 601288 Human
    • SwissProt: P63104 Human
    • Unigene: 492407 Human
    • Alternative names

      • 14 3 3 delta antibody
      • 14 3 3 protein zeta/delta antibody
      • 14 3 3 protein/cytosolic phospholipase A2 antibody
      • 14 3 3 zeta antibody
      • 14-3-3 protein zeta/delta antibody
      • 1433Z_HUMAN antibody
      • Epididymis luminal protein 4 antibody
      • Epididymis secretory protein Li 3 antibody
      • HEL S 3 antibody
      • HEL4 antibody
      • KCIP-1 antibody
      • KCIP1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • Phospholipase A2 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • YWHAD antibody
      • YWHAZ antibody
      see all

    Images

    • Western blot - Anti-14-3-3 zeta antibody (ab51129)
      Western blot - Anti-14-3-3 zeta antibody (ab51129)
      All lanes : Anti-14-3-3 zeta antibody (ab51129)

      Lane 1 : Extracts from 293 cells treated
      with Forskolin (40nM, 30min) with no peptide
      Lane 2 : Extracts from 293 cells treated
      with Forskolin (40nM, 30min) with immunising peptide

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm

    • Western blot - Anti-14-3-3 zeta antibody (ab51129)
      Western blot - Anti-14-3-3 zeta antibody (ab51129)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)
      This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
    • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)
      Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)
      ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (20)

    Publishing research using ab51129? Please let us know so that we can cite the reference in this datasheet.

    ab51129 has been referenced in 20 publications.

    • Shi J  et al. YWHAZ promotes ovarian cancer metastasis by modulating glycolysis. Oncol Rep 41:1101-1112 (2019). PubMed: 30535456
    • Li J  et al. 14-3-3? promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling. Cancer Med 8:783-794 (2019). PubMed: 30656845
    • Liu J  et al. Circ-SERPINE2 promotes the development of gastric carcinoma by sponging miR-375 and modulating YWHAZ. Cell Prolif 52:e12648 (2019). PubMed: 31199037
    • Fletcher CE  et al. Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer. Oncogene 38:5700-5724 (2019). PubMed: 31043708
    • Tomati V  et al. High-throughput screening identifies FAU protein as a regulator of mutant cystic fibrosis transmembrane conductance regulator channel. J Biol Chem 293:1203-1217 (2018). PubMed: 29158263
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-14-3-3 zeta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast Cancer cell line (MCF7))
    Loading amount
    20 µg
    Specification
    Breast Cancer cell line (MCF7)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2011

    Western blot abreview for Anti-14-3-3 zeta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophage)
    Loading amount
    20 µg
    Specification
    macrophage
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2010

    Western blot abreview for Anti-14-3-3 zeta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Colorectal cancer)
    Loading amount
    30 µg
    Specification
    Colorectal cancer
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Lay Harn Gam

    Verified customer

    Submitted May 19 2010

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