Anti-14-3-3 zeta antibody (ab51129)
- Datasheet
- References (19)
- Protocols
Overview
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Product name
Anti-14-3-3 zeta antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta -
Host species
Rabbit -
Specificity
ab51129 detects endogenous levels of total 14-3-3 zeta protein. -
Tested applications
Suitable for: ELISA, WB, ICC, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
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Positive control
- Human breast carcinoma tissue or extracts from 293 cells treated with Forskolin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/40000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). | |
| ICC | Use a concentration of 1 µg/ml. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/50 - 1/100. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
Cellular localization
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Entrez Gene: 25578 Rat
- Omim: 601288 Human
- SwissProt: P63104 Human
- SwissProt: P63101 Mouse
- SwissProt: P63102 Rat
- Unigene: 492407 Human
see all -
Alternative names
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
see all
Images
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Western blot - Anti-14-3-3 zeta antibody (ab51129)All lanes : Anti-14-3-3 zeta antibody (ab51129)
Lane 1 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with immunising peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm
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Western blot - Anti-14-3-3 zeta antibody (ab51129) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Shi J et al. YWHAZ promotes ovarian cancer metastasis by modulating glycolysis. Oncol Rep 41:1101-1112 (2019). Read more (PubMed: 30535456) »
- Li J et al. 14-3-3? promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling. Cancer Med 8:783-794 (2019). Read more (PubMed: 30656845) »
