Anti-14-3-3 zeta antibody (ab51129)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 zeta antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta -
Host species
Rabbit -
Specificity
ab51129 detects endogenous levels of total 14-3-3 zeta protein.
Cross reactivity of ab51129 antibody with other member of the 14-3-3 family:
-92% identities with 14-3-3 beta/alpha, gamma and eta
-77% identities with 14-3-3 teta
-69% identities with 14-3-3 epsilon
-62% identities with 14-3-3 sigma -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human 14-3-3 zeta. The immunogen is a 13 amino acid synthetic peptide that resides within amino acids 24-73 of the human 14-3-3 zeta.
Database link: P63104 -
Positive control
- WB: 293 cells IHC-P: Human glioblastoma, human breast carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
1/50 - 1/100. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
Cellular localization
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 7534 Human
- Omim: 601288 Human
- SwissProt: P63104 Human
- Unigene: 492407 Human
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Alternative names
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
see all
Images
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Western blot - Anti-14-3-3 zeta antibody (ab51129)All lanes : Anti-14-3-3 zeta antibody (ab51129)
Lane 1 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with immunising peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm
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Western blot - Anti-14-3-3 zeta antibody (ab51129) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (20)
ab51129 has been referenced in 20 publications.
- Shi J et al. YWHAZ promotes ovarian cancer metastasis by modulating glycolysis. Oncol Rep 41:1101-1112 (2019). PubMed: 30535456
- Li J et al. 14-3-3? promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling. Cancer Med 8:783-794 (2019). PubMed: 30656845
- Liu J et al. Circ-SERPINE2 promotes the development of gastric carcinoma by sponging miR-375 and modulating YWHAZ. Cell Prolif 52:e12648 (2019). PubMed: 31199037
- Fletcher CE et al. Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer. Oncogene 38:5700-5724 (2019). PubMed: 31043708
- Tomati V et al. High-throughput screening identifies FAU protein as a regulator of mutant cystic fibrosis transmembrane conductance regulator channel. J Biol Chem 293:1203-1217 (2018). PubMed: 29158263
