Anti-14-3-3 zeta antibody (ab51129)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 zeta antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta -
Host species
Rabbit -
Specificity
ab51129 detects endogenous levels of total 14-3-3 zeta protein.
Cross reactivity of ab51129 antibody with other member of the 14-3-3 family:
-92% identities with 14-3-3 beta/alpha, gamma and eta
-77% identities with 14-3-3 teta
-69% identities with 14-3-3 epsilon
-62% identities with 14-3-3 sigma -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human 14-3-3 zeta. The immunogen is a 13 amino acid synthetic peptide that resides within amino acids 24-73 of the human 14-3-3 zeta.
Database link: P63104 -
Positive control
- WB: 293 cells IHC-P: Human glioblastoma, human breast carcinoma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
1/50 - 1/100. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
Cellular localization
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 7534 Human
- Omim: 601288 Human
- SwissProt: P63104 Human
- Unigene: 492407 Human
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Alternative names
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
see all
Images
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Western blot - Anti-14-3-3 zeta antibody (ab51129)All lanes : Anti-14-3-3 zeta antibody (ab51129)
Lane 1 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from 293 cells treated
with Forskolin (40nM, 30min) with immunising peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm
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Western blot - Anti-14-3-3 zeta antibody (ab51129) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab51129)This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab51129)ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (26)
ab51129 has been referenced in 26 publications.
- Wang D et al. Loss of 4.1N in epithelial ovarian cancer results in EMT and matrix-detached cell death resistance. Protein Cell 12:107-127 (2021). PubMed: 32448967
- Gong Y et al. MiRNA-1225 Inhibits Osteosarcoma Tumor Growth and Progression by Targeting YWHAZ. Onco Targets Ther 14:15-27 (2021). PubMed: 33442263
- Hu S et al. Radiation-induced abscopal reproductive effect is driven by TNF-a/p38 MAPK/Rac1 axis in Sertoli cells. Theranostics 11:5742-5758 (2021). PubMed: 33897879
- Hui C et al. Circular RNA circNHSL1 Contributes to Gastric Cancer Progression Through the miR-149-5p/YWHAZ Axis. Cancer Manag Res 12:7117-7130 (2020). PubMed: 32848466
- Liu Y et al. Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/YWHAZ Axis. Onco Targets Ther 13:9377-9388 (2020). PubMed: 33061425
