• Product name

    Anti-14-3-3 zeta (phospho S58) antibody
    See all 14-3-3 zeta primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 zeta (phospho S58)
  • Host species

  • Specificity

    14-3-3 zeta (phospho S58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58. The immunogen sequence shows 92% homology with 14-3-3 gamma, eta, and beta/alpha. There may be cross reactivity with these other proteins.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide from Human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-phosphoS-W-R).

  • Positive control

    • NIH/3T3 cell extract; paraffin-embedded human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab51109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational

    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localization

    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all


  • All lanes : Anti-14-3-3 zeta (phospho S58) antibody (ab51109) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract (treated with UV for 30 mins) with phosphopeptide immunogen (phospho S58)
    Lane 2 : NIH/3T3 cell extract (treated with UV for 30 mins)

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Ab51109 staining human 14-3-3 zeta in human breast carcinoma tissue in immunohistochemistry using paraffin embedded tissue.
  • ICC/IF image of ab51109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Kaplan A  et al. Small-Molecule Stabilization of 14-3-3 Protein-Protein Interactions Stimulates Axon Regeneration. Neuron 93:1082-1093.e5 (2017). Read more (PubMed: 28279353) »
  • Lachaud C  et al. 14-3-3-Regulated Ca(2+)-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis. Cell Death Differ : (2012). Arabidopsis thaliana . Read more (PubMed: 22935611) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Loading amount
100 µg
Gel Running Conditions
Non-reduced Denaturing
Arabidopsis thaliana Purified protein (suspension cells)
suspension cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 01 2013


Thank you for contacting Abcam regarding ab51109. I have confirmed that the immunogen sequence for this antibody is XXX. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More
Western blot
Arabidopsis thaliana Purified protein (Arabidopsis suspension cell culture)
Loading amount
1.5 µg
Arabidopsis suspension cell culture
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: r.t°C

Abcam user community

Verified customer

Submitted Dec 10 2010

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