Key features and details
- Rabbit polyclonal to 14-3-3 zeta (phospho S58)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-14-3-3 zeta (phospho S58) antibody
See all 14-3-3 zeta primary antibodies
DescriptionRabbit polyclonal to 14-3-3 zeta (phospho S58)
Specificity14-3-3 zeta (phospho S58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58. The immunogen sequence shows 92% homology with 14-3-3 gamma, eta, and beta/alpha. There may be cross reactivity with these other proteins.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide from Human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-phosphoS-W-R).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab51109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
Cellular localizationCytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
- Information by UniProt
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
All lanes : Anti-14-3-3 zeta (phospho S58) antibody (ab51109) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (treated with UV for 30 mins) with phosphopeptide immunogen (phospho S58)
Lane 2 : NIH/3T3 cell extract (treated with UV for 30 mins)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Ab51109 staining human 14-3-3 zeta in human breast carcinoma tissue in immunohistochemistry using paraffin embedded tissue.
ICC/IF image of ab51109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51109 has been referenced in 4 publications.
- Kaplan A et al. Small-Molecule Stabilization of 14-3-3 Protein-Protein Interactions Stimulates Axon Regeneration. Neuron 93:1082-1093.e5 (2017). PubMed: 28279353
- Lachaud C et al. 14-3-3-Regulated Ca(2+)-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis. Cell Death Differ : (2012). Arabidopsis thaliana . PubMed: 22935611
- Liu F et al. Shiga toxin (Stx)1B and Stx2B induce von Willebrand factor secretion from human umbilical vein endothelial cells through different signaling pathways. Blood 118:3392-8 (2011). WB ; Human . PubMed: 21816831
- Koseki N et al. 17ß-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein levels following a neurotoxic insult. Brain Res : (2011). WB ; Rat . PubMed: 22154405