Key features and details
- Sensitivity: 3 pg/ml
- Range: 0.1 ng/ml - 1000 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Product name14,15 DHET ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 12 0.93% Inter-assay Sample n Mean SD CV% Overall 12 1.31%
Sample typeUrine, Serum, Plasma, Cell culture extracts, Tissue
Sensitivity= 3 pg/ml
Range0.1 ng/ml - 1000 ng/ml
Assay durationMultiple steps standard assay
Abcam's 14,15 DHET competitive in vitro ELISA Kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture, tissue samples following proper isolation and purification.
This competitive ELISA kit is based on competition between the 14,15-DHET epitope and the 14,15-DHET-HRP conjugate for a limited number of binding sites available from the anti-14,15-DHET antibody; which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 14,15-DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 14,15-DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 14,15-DHET in the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.
This competitive ELISA kit is for determination of 14,15-DHET levels in biological samples. Specificity of the ELISA has been published. The 14,15-DHET level exhibited strong positive correlation with hypertension, brain injury and stroke in rodents. 14,15-DHET is a representative metabolite of soluble epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochromes P450. Human blood 14,15-DHET levels were measured using the 14,15-DHET ELISA kit. Increased 14,15-DHET levels of human cells as detected by the 14,15-DHET ELISA were indicative of the neoplastic and metastatic phenotype of carcinoma cells.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 14,15-DHET Standard 1 x 2µl 14,15-DHET-HRP Conjugates 1 x 12µl HRP Buffer 1 x 15ml Pre-coated 96-Well Microplate 1 unit 10X Sample Dilution Buffer 1 x 25ml TMB Substrate 1 x 24ml 10X Wash Buffer Solution 1 x 25ml
- 11,12-dihydroxy-5Z,8Z,14Z-eicosatrienoic acid
ab175811 has been referenced in 1 publication.
- Gai Z et al. Effects of Farnesoid X Receptor Activation on Arachidonic Acid Metabolism, NF-kB Signaling, and Hepatic Inflammation. Mol Pharmacol 94:802-811 (2018). PubMed: 29743187