Overview

  • Product name
    14,15 DHET sEH (soluble Epoxide Hydrolase) ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 12 0.93%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12 1.31%
  • Sample type
    Urine, Serum, Plasma, Tissue, Cell culture media
  • Assay type
    Competitive
  • Sensitivity
    3 pg/ml
  • Range
    0.01 ng/ml - 1000 ng/ml
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s 14,15 DHET sEH competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate the quantitative measurement in biological samples and cell culture media following proper isolation and purification.

     

    ab175816  is a metabolite formed by the soluble epoxide hydrolase (sEH)-mediated metabolism of 14,15-EET which itself is generated by arachidonic acid epoxygenase activity of cytochromes P450 (CYPs) 2C and 2J. This competitive ELISA kit with an HRP detection system can been used to determine 14,15-DHET levels in biological samples(tissue, plasma, and urine) and cell culture media as a measure of sEH activity. In addition, this assay can also be used to screen for inhibitors of soluble epoxide hydrolase activity. The kit is similar to our 14,15-DHET/EET ELISA kit (DH 2) but also contains the EET substrate. Each kit for a 96-well plate is good for triplicate analyses of up to 12 samples. 

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab175816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab175816 - 14,15 DHET sEH (soluble Epoxide Hydrolase) ELISA Kit.

Protocols

References

ab175816 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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