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    1415-eet--dhet-elisa-kit-ab175812.pdf

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Signal Transduction Signaling Pathway Lipid Signaling Other
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14,15 EET / DHET ELISA Kit (ab175812)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (2)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 3 pg/ml
    • Range: 0.01 ng/ml - 1000 ng/ml
    • Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
    • Detection method: Colorimetric
    • Assay type: Competitive

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    Overview

    • Product name

      14,15 EET / DHET ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      overall 12 0.93%
      Inter-assay
      Sample n Mean SD CV%
      overall 12 1.31%
    • Sample type

      Urine, Serum, Plasma, Cell culture extracts, Tissue
    • Assay type

      Competitive
    • Sensitivity

      3 pg/ml
    • Range

      0.01 ng/ml - 1000 ng/ml
    • Assay duration

      Multiple steps standard assay
    • Product overview

      Abcam’s 14,15 EET / DHET in vitro ELISA kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture, tissue samples following proper isolation and purification.

       

      A 96-well plate has been precoated with 14,15-DHET antibody. Samples and the 14,15-DHET-HRP conjugate are added to the wells, where 14,15-DHETin the sample competes with the added 14,15-DHET-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 14,15-DHET in the sample and the intensity is measured at 450 nm.

      It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo. Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. In human carcinoma cells, rAAV-mediated over expression of CYP2J2 resulted in a marked increase in 14,15-DHET level in cell plasma, whereas rAAV-anti2J2-mediated silence of CYP2J2 expression significantly decreased its production. Abcam’s14,15EET/DHET in vitro ELISA kit can be used to measure EET levels in cultured cells which express sEH.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      14,15-DHET Standard 1 x 2µl
      14,15-DHET-HRP Conjugates 1 x 12µl
      HRP Buffer 1 x 15ml
      Pre-coated 96-Well Microplate 1 unit
      10X Sample Dilution Buffer 1 x 25ml
      TMB Substrate 1 x 24ml
      10X Wash Buffer Solution 1 x 25ml
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Other
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • STATs
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • STATs
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Vasculature
      • Vasodilation
      • Other
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
    • Alternative names

      • 14,15-dihydroxy-5Z,8Z,11Z-eicosatrienoic acid

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab175812 14,15 EET/DHET ELISA Kit.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab175812? Please let us know so that we can cite the reference in this datasheet.

    ab175812 has been referenced in 2 publications.

    • Dai N  et al. The Anti-inflammatory Effect of Soluble Epoxide Hydrolase Inhibitor and 14, 15-EET in Kawasaki Disease Through PPAR?/STAT1 Signaling Pathway. Front Pediatr 8:451 (2020). PubMed: 32903307
    • Li H  et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228

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