Recombinant
RabMAb

Recombinant Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Overview

  • Product name
    Anti-15 Lipoxygenase 1 antibody [EPR22138]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human 15 Lipoxygenase 1 aa 100-650. The exact sequence is proprietary.
    Database link: P16050

  • Positive control
    • WB: HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate; Mouse and rat lung lysates; Human colon lysate. IHC-P: Human colon and bronchus tissues; Mouse colon tissue; Rat bronchus tissue. IHC-Fr: Mouse colon tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab244205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr 1/500.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P 1/4000.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
    • Pathway
      Lipid metabolism; leukotriene D4 biosynthesis.
    • Sequence similarities
      Belongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 15 lipoxygenase 1 antibody
      • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
      • 15 LOX antibody
      • 15 LOX 1 antibody
      • 15-LOX antibody
      • 15LOX 1 antibody
      • ALOX15 antibody
      • Arachidonate 15 lipoxygenase antibody
      • Arachidonate 15-lipoxygenase antibody
      • Arachidonate omega 6 lipoxygenase antibody
      • Arachidonate omega-6 lipoxygenase antibody
      • LOG15 antibody
      • LOX15_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in eosinophils of mouse colon (PMID:23503644).

      The nuclear counter stain is DAPI (blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate
      Lane 3 : Human colon lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa



      Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 15 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • Immunohistochemical analysis of paraffin-embedded rat bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of rat bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of mouse colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded human bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of human bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    References

    ab244205 has not yet been referenced specifically in any publications.

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