Overview

  • Product name

    15-deoxy-delta12,14-PGJ2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 16 1136pg/ml 5.6%
    Medium 16 3062pg/ml 5.7%
    High 16 4394pg/ml 7.4%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 1195pg/ml 15.7%
    Medium 8 4406pg/ml 13%
    High 8 7219pg/ml 14.5%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Plasma
  • Assay type

    Competitive
  • Sensitivity

    36.8 pg/ml
  • Range

    195 pg/ml - 200000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 94.4 % - %
    Urine 113.9 % - %
    Plasma 105 % - %
    Tissue Culture Media 97.4 % - %

  • Assay time

    5h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s 15-deoxy-delta12,14-PGJ2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-deoxy-delta12,14-PGJ2 in tissue culture media, Human saliva, Human urine and porcine plasma.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15-deoxy-delta12,14-PGJ2 antigen and a polyclonal rabbit antibody specific to 15-deoxy-delta12,14-PGJ2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15-deoxy-delta12,14-PGJ2 captured in the plate.

  • Notes

    15-deoxy-Δ12,14-PGJ2 is one of the ultimate dehydration products of PGD2. PGD2 is formed from PGH2 which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. In the aqueous solution PGD2 forms PGJ2. Albumin or other serum proteins present in the sample will convert PGD2 to the isomeric compound, Δ12-PGJ2. In males about 152 ng of Δ12-PGJ2 is excreted in urine per day, and in women about half this amount is excreted. 15-deoxy-Δ12,14-PGJ2 has been shown as an inducer of adipogenesis and an activator of the γ isoform of the peroxisome proliferation activated receptor (PPARγ).

    Cross Reactivity 

    CompoundCross Reactivity
    15-deoxy-Δ12,14-PGJ2100%
    PGJ249.2%
    Δ12-PGJ25.99%
    PGD24.92%
    Arachidonic Acid0.03%
    PGE2<0.01%
    PGF<0.01%
    9α,11β-PGF<0.01%
    Thromboxane B2<0.01%
    2-Arachidonoylglycerol<0.01%
    Anandamide<0.01%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133031.

Protocols

References

This product has been referenced in:

  • Ito M  et al. Prostanoid-dependent spontaneous pain and PAR2-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4. Mol Pain 13:1744806917704138 (2017). Read more (PubMed: 28381109) »
  • Thomas GW  et al. The low molecular weight fraction of human serum albumin upregulates production of 15d-PGJ2 in Peripheral Blood Mononuclear Cells. Biochem Biophys Res Commun 473:1328-1333 (2016). Read more (PubMed: 27095392) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

ab133031, PGJ2 ELISA Kit, mouse

Good Good 4/5 (Ease of Use)
Abreviews
We were pleased that your ELISA kit was able to detect PGJ2 secretion using mouse cell supernatants.

Abcam user community

Verified customer

Submitted Feb 27 2018

Question
Answer

Te pido disculpas por la espera.

Acabo de recibir la respuesta de mis compañeros acerca de la proteína 15-deoxy-delta12,14-PGJ2 del kit ab133031 que os interesaba.

Lamentablemente me confirman que no puede venderse la proteína estándar por separado.

Siento mucho no poder ayudarte en esta ocasión. Si tuvieras cualquier otra consulta, por favor no dudes en volvernos a contactar.

Que pases un buen día.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up