Product name15-deoxy-delta12,14-PGJ2 ELISA Kit
Intra-assay Sample n Mean SD CV% Low 16 1136pg/ml 5.6% Medium 16 3062pg/ml 5.7% High 16 4394pg/ml 7.4% Inter-assay Sample n Mean SD CV% Low 8 1195pg/ml 15.7% Medium 8 4406pg/ml 13% High 8 7219pg/ml 14.5%
Sample typeCell culture supernatant, Saliva, Urine, Plasma
Range195 pg/ml - 200000 pg/ml
Sample specific recovery Sample type Average % Range Saliva 94.4 % - % Urine 113.9 % - % Plasma 105 % - % Tissue Culture Media 97.4 % - %
Assay time5h 0m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human, Pig
Predicted to work with: a wide range of other species
Abcam’s 15-deoxy-delta12,14-PGJ2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-deoxy-delta12,14-PGJ2 in tissue culture media, Human saliva, Human urine and porcine plasma.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15-deoxy-delta12,14-PGJ2 antigen and a polyclonal rabbit antibody specific to 15-deoxy-delta12,14-PGJ2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15-deoxy-delta12,14-PGJ2 captured in the plate.
15-deoxy-Δ12,14-PGJ2 is one of the ultimate dehydration products of PGD2. PGD2 is formed from PGH2 which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. In the aqueous solution PGD2 forms PGJ2. Albumin or other serum proteins present in the sample will convert PGD2 to the isomeric compound, Δ12-PGJ2. In males about 152 ng of Δ12-PGJ2 is excreted in urine per day, and in women about half this amount is excreted. 15-deoxy-Δ12,14-PGJ2 has been shown as an inducer of adipogenesis and an activator of the γ isoform of the peroxisome proliferation activated receptor (PPARγ).
Compound Cross Reactivity 15-deoxy-Δ12,14-PGJ2 100% PGJ2 49.2% Δ12-PGJ2 5.99% PGD2 4.92% Arachidonic Acid 0.03% PGE2 <0.01% PGF2α <0.01% 9α,11β-PGF2α <0.01% Thromboxane B2 <0.01% 2-Arachidonoylglycerol <0.01% Anandamide <0.01%
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 15-d-PGJ2 Alkaline Phosphatase Conjugate 1 x 5ml 15-d-PGJ2 Standard 1 x 0.5ml 20X Wash Buffer Concentrate 1 x 27ml Anti-15-d-PGJ2 Antibody 1 x 5ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
- Prostaglandin J2
Our Abpromise guarantee covers the use of ab133031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Ito M et al. Prostanoid-dependent spontaneous pain and PAR2-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4. Mol Pain 13:1744806917704138 (2017). Read more (PubMed: 28381109) »
- Thomas GW et al. The low molecular weight fraction of human serum albumin upregulates production of 15d-PGJ2 in Peripheral Blood Mononuclear Cells. Biochem Biophys Res Commun 473:1328-1333 (2016). Read more (PubMed: 27095392) »