15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist (ab141717)

Overview

  • Product name

    15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist
  • Description

    Selective PPARγ agonist
  • Biological description

    Selective agonist to PPARγ. Inhibits the proliferation of cancer cell lines (IC50 = 22 μM). Differentiates C3H10T1/2 fibroblasts into adipocytes. Active in vitro.
  • Purity

    > 97%
  • CAS Number

    87893-55-8
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (Z)-7-[(1S,5E)-5-[(E)-Oct-2-enylidene]-4-oxocyclopent-2-en-1-yl]hept-5-enoic acid
  • Molecular weight

    316.50
  • Molecular formula

    C20H28O3
  • PubChem identifier

    5311211
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 75 mM and in ethanol to 75 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCCCCC=CC=C1C(C=CC1=O)CC=CCCCC(=O)O
  • Source

    Synthetic

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab141717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).

References

This product has been referenced in:

  • Paulitschke V  et al. Proteome analysis identified the PPAR? ligand 15d-PGJ2 as a novel drug inhibiting melanoma progression and interfering with tumor-stroma interaction. PLoS One 7:e46103 (2012). Read more (PubMed: 23049949) »
  • Itoh T  et al. Structural basis for the activation of PPARgamma by oxidized fatty acids. Nat Struct Mol Biol 15:924-31 (2008). Read more (PubMed: 19172745) »
  • Kliewer SA  et al. A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor gamma and promotes adipocyte differentiation. Cell 83:813-9 (1995). Read more (PubMed: 8521498) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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