Overview

  • Product name

    Anti-15 Lipoxygenase 1 antibody [7H6]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description

    Mouse monoclonal [7H6] to 15 Lipoxygenase 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human 15 Lipoxygenase 1. Produced in HEK-293T cells (NP_001131).
    Database link: P16050

  • Positive control

    • IHC-P: Human lung and colon tissues. ICC/IF: COS-7 cells. WB: pCMV6-ENTRY ALOX15-transfected HEK-293T cell lysate. Flow Cyt: HEK-293T cells transfected with ALOX15 overexpress plasmid.

Properties

Applications

Our Abpromise guarantee covers the use of ab230919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
ICC/IF 1/100.
Flow Cyt 1/100.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway

    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 15 lipoxygenase 1 antibody
    • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
    • 15 LOX antibody
    • 15 LOX 1 antibody
    • 15-LOX antibody
    • 15LOX 1 antibody
    • ALOX15 antibody
    • Arachidonate 15 lipoxygenase antibody
    • Arachidonate 15-lipoxygenase antibody
    • Arachidonate omega 6 lipoxygenase antibody
    • Arachidonate omega-6 lipoxygenase antibody
    • LOG15 antibody
    • LOX15_HUMAN antibody
    see all

Images

  • All lanes : Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY 15 Lipoxygenase 1-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.
  • Formalin-fixed, paraffin-embedded human colon tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.

  • pCMV6-ENTRY 15 Lipoxygenase 1-transiently transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for 15 Lipoxygenase 1 (green) using ab230919 at 1/100 dilution in ICC/IF.

  • Flow cytometric analysis of overexpression plasmid-transfected HEK-293T (human breast adenocarcinoma cell line) cells labeling 15 Lipoxygenase 1 with ab230919 at 1/100 dilution (red) compared with an empty vector control-transfected HEK-293T cell line (blue).

References

ab230919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230919.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up