Overview

  • Product name

    Anti-15 Lipoxygenase 1 antibody
    See all 15 Lipoxygenase 1 primary antibodies
  • Description

    Rabbit polyclonal to 15 Lipoxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human 15 Lipoxygenase 1 aa 610-643 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 246

  • Positive control

    • Ramos cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF 1/10 - 1/50.
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA 1/1000.

Target

  • Function

    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway

    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 15 lipoxygenase 1 antibody
    • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
    • 15 LOX antibody
    • 15 LOX 1 antibody
    • 15-LOX antibody
    • 15LOX 1 antibody
    • ALOX15 antibody
    • Arachidonate 15 lipoxygenase antibody
    • Arachidonate 15-lipoxygenase antibody
    • Arachidonate omega 6 lipoxygenase antibody
    • Arachidonate omega-6 lipoxygenase antibody
    • LOG15 antibody
    • LOX15_HUMAN antibody
    see all

Images

  • All lanes : Anti-15 Lipoxygenase 1 antibody (ab80221)

    Lane 1 : Hela cell line lysate
    Lane 2 : A549 cell line lysate
    Lane 3 : K562 cell line lysate
    Lane 4 : MDA-MB-435 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 73 kDa

  • ab80221 at 1/50 dilution staining 15 Lipoxygenase 1 - C-terminal in human lymph tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ab80221 staining 15 Lipoxygenase 1 in HeLa celss by ICC/IF (Immunocytochemistry/immunofluorescence). An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. Cell nuclear was stained blue with DAPI

  • Anti-15 Lipoxygenase 1 antibody (ab80221) at 1/50 dilution + Ramos cell line lysate at 35 µg

    Predicted band size: 73 kDa
    Observed band size: 73 kDa
    Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Han YH  et al. A maresin 1/RORa/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis. J Clin Invest 130:1684-1698 (2019). Read more (PubMed: 30855276) »
  • Shi H  et al. A regulatory role for ß-adrenergic receptors regarding the resolvin D1 (RvD1) pathway in the diabetic retina. PLoS One 12:e0185383 (2017). WB . Read more (PubMed: 29095817) »
See all 4 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cecum tissue)
Permeabilization
Yes - 0.5% Tx-100 in PBS, 5 min
Specification
cecum tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 18 2019

Answer

Thank you for submitting an Abreview for ab80221. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- try a different fixation reagent (e.g. acetone)
- increase the permeabilization time
- use more primary antibody (e.g. 1/50)
If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 21 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 06 2012

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Answer

Thank you for your reply.

I am sorry to hear that despite some protocol modifications, this antibody is still not producing the expected result. I would be happy to offer a replacement or credit. We have several other Anti-15 Lipoxygenase 1 antibodies that have been validated for WB with human samples, including ab54772, ab80495, ab110100, and ab119774. Please check the datasheets to see if you would like to try one of these.

I look forward to your reply so that I may assist you further and I apologize for the inconvenience caused. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for your reply with the requested information.

Can I confirm that you are not detecting any bands with the conditions that you have specified? Based on our testing, I would recommend increasing the amount of protein loaded to 60ug rather than 30ug, testing a dilution of 1:50. This should enhance the signal for better detection. Your other protocol steps seem to be fine and are in line with what we would recommend for this product.

I hope this information is helpful. If these suggestions do not improve your results or if you have any additional questions, please do not hesitate to contact me.

Read More

Answer

Thank you for contacting Abcam regarding ab80221. I am sorry that you have been experiencing difficulties using this antibody. In order to assist you further and provide some protocol suggestions, can you please specify which application you are using? Also, if you would provide a brief summary of your protocol and sample preparation, I would be happy to offer technical advice. If I am not able to offer suggestions to improve your results with this antibody, I would happy to offer a replacement or credit per our Abpromise guarantee. I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 16 2011

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