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Please find attached an outline of the Protocol which I have been using with ab80221 I would appreciate any technical information you could provide.
Asked on Jan 16 2012
Thank you for your reply with the requested information.
Can I confirm that you are not detecting any bands with the conditions that you have specified? Based on our testing, I would recommend increasing the amount of protein loaded to 60ug rather than 30ug, testing a dilution of 1:50. This should enhance the signal for better detection. Your other protocol steps seem to be fine and are in line with what we would recommend for this product.
I hope this information is helpful. If these suggestions do not improve your results or if you have any additional questions, please do not hesitate to contact me.
Answered on Jan 16 2012