Question (33139) | Anti-15 Lipoxygenase 1 antibody (ab80221)

Go to datasheet (ab80221)

Question

Please find attached an outline of the Protocol which I have been using with ab80221 I would appreciate any technical information you could provide.

Answer

Thank you for your reply with the requested information.

Can I confirm that you are not detecting any bands with the conditions that you have specified? Based on our testing, I would recommend increasing the amount of protein loaded to 60ug rather than 30ug, testing a dilution of 1:50. This should enhance the signal for better detection. Your other protocol steps seem to be fine and are in line with what we would recommend for this product.

I hope this information is helpful. If these suggestions do not improve your results or if you have any additional questions, please do not hesitate to contact me.

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