Question (51844) | Anti-15 Lipoxygenase 1 antibody (ab80221)

Go to datasheet (ab80221)

Question

ReviewID: xxx AbID: 80221, Anti-15 Lipoxygenase 1 antibody - C-terminal Rating: Below Average Image: Anti-15 Lipoxygenase 1 antibody - C-terminal for Immunocytochemistry/ Immunofluorescence (Mouse) Sample: Species: Mouse Type: Cell Specification: RAW 264.7 Application: Application: Fixative: Paraformaldehyde Permeabilization: Yes Blocking step: BSA as blocking agent for 1 hour at 22°C Other detail: Dilution: 1/100 Incubation time: 16 hours at 4°C Diluent: 2% BSA in PBS Secondary Antibody: Name: Non-Abcam Antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L) Conjugation: Alexa Fluor® 488 Dilution: 1/1000 Additional Data:

Answer

Thank you for submitting an Abreview for ab80221. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- try a different fixation reagent (e.g. acetone)
- increase the permeabilization time
- use more primary antibody (e.g. 1/50)
If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

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