Immunocytochemistry/ Immunofluorescence abreview for Anti-15 Lipoxygenase 1 antibody - C-terminal

Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Abcam response
Thank you so much for testing this antibody in ICC/IF.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this application. We would welcome additional feedback from other customers as well.

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 21 2012

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