Anti-15 Lipoxygenase 1 antibody (ab80495)
Key features and details
- Goat polyclonal to 15 Lipoxygenase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-15 Lipoxygenase 1 antibody
See all 15 Lipoxygenase 1 primary antibodies -
Description
Goat polyclonal to 15 Lipoxygenase 1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Synthetic peptide:
C-HYKTDVAVKDDPE
, corresponding to internal sequence amino acids 482-494 of Human 15 Lipoxygenase 1 (NP_001131.3). -
Positive control
- Nuclear HeLa cell lysate and whole Hela lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab80495 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
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WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. -
Pathway
Lipid metabolism; leukotriene D4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 246 Human
- Entrez Gene: 100174413 Orangutan
- Omim: 152392 Human
- SwissProt: P16050 Human
- SwissProt: Q5RBE8 Orangutan
- Unigene: 73809 Human
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Alternative names
- 15 lipoxygenase 1 antibody
- 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
- 15 LOX antibody
see all
Images
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Anti-15 Lipoxygenase 1 antibody (ab80495) at 0.2 µg/ml + nuclear HeLa cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab80495 has not yet been referenced specifically in any publications.