Recombinant
RabMAb

Recombinant Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062)

Overview

  • Product name

    Anti-15 Lipoxygenase 1 antibody [EPR22136]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22136] to 15 Lipoxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 15 Lipoxygenase 1 aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: P16050

  • Positive control

    • WB: 15 Lipoxygenase 1 (WT) transfected HEK-293T whole cell lysate. Human colon lysate. IHC-P: Human bronchia and colon tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab242062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 75 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
    • Pathway

      Lipid metabolism; leukotriene D4 biosynthesis.
    • Sequence similarities

      Belongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 15 lipoxygenase 1 antibody
      • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
      • 15 LOX antibody
      • 15 LOX 1 antibody
      • 15-LOX antibody
      • 15LOX 1 antibody
      • ALOX15 antibody
      • Arachidonate 15 lipoxygenase antibody
      • Arachidonate 15-lipoxygenase antibody
      • Arachidonate omega 6 lipoxygenase antibody
      • Arachidonate omega-6 lipoxygenase antibody
      • LOG15 antibody
      • LOX15_HUMAN antibody
      see all

    Images

    • Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/1000 dilution + Human colon at 20 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 75 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?


      Exposure time: 114 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 75 kDa


      Exposure time: 37 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • Immunohistochemical analysis of paraffin-embedded human bronchia tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in epithelial cells of human bronchia (PMID:24036245; PMID:21831839) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab242062 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    References

    ab242062 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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