Name: Anti-15 Lipoxygenase 1 antibody [EPR22138]
Brand: RabMAb
SKU: ab244205
Rating: 4 (1 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
Suitable for: WB, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-15 Lipoxygenase 1 antibody [EPR22138]
See all 15 Lipoxygenase 1 primary antibodies
Description

Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate; Mouse and rat lung lysates; Human colon lysate. IHC-P: Human colon and bronchus tissues; Mouse colon tissue; Rat bronchus tissue. IHC-Fr: Mouse colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22138
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
- Anti-15 Lipoxygenase 1 antibody [EPR22138] - BSA and Azide free (ab245201)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Recombinant Protein
- Recombinant Human 15 Lipoxygenase 1 protein (ab114421)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr		1/500. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr 1/500. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
Pathway

Lipid metabolism; leukotriene D4 biosynthesis.
Sequence similarities

Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P16050 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 246 Human
- Entrez Gene: 11687 Mouse
- Entrez Gene: 81639 Rat
- Omim: 152392 Human
- SwissProt: P16050 Human
- SwissProt: P39654 Mouse
- SwissProt: Q02759 Rat
- Unigene: 73809 Human
Alternative names
- 15 lipoxygenase 1 antibody
- 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
- 15 LOX antibody
- 15 LOX 1 antibody
- 15-LOX antibody
- 15LOX 1 antibody
- ALOX15 antibody
- Arachidonate 15 lipoxygenase antibody
- Arachidonate 15-lipoxygenase antibody
- Arachidonate omega 6 lipoxygenase antibody
- Arachidonate omega-6 lipoxygenase antibody
- LOG15 antibody
- LOX15_HUMAN antibody
see all

Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in eosinophils of mouse colon (PMID:23503644).

The nuclear counter stain is DAPI (blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Human colon lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded rat bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of rat bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of mouse colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded human bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of human bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab244205? Please let us know so that we can cite the reference in this datasheet.

ab244205 has been referenced in 1 publication.

Tian Q et al. Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma. Front Cell Dev Biol 9:684259 (2021). PubMed: 34307361

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (macrophage)

Permeabilization

Yes - 0.1%triton x100

Specification

macrophage

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

DR. Prashant Rai

Verified customer

Submitted Aug 31 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Pathway

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-15 Lipoxygenase 1 antibody [EPR22138]