RecombinantRabMAb

Recombinant Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

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Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-15 Lipoxygenase 1 antibody [EPR22138]
    See all 15 Lipoxygenase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate; Mouse and rat lung lysates; Human colon lysate. IHC-P: Human colon and bronchus tissues; Mouse colon tissue; Rat bronchus tissue. IHC-Fr: Mouse colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr
1/500.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr
1/500.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.

    • Pathway

      Lipid metabolism; leukotriene D4 biosynthesis.

    • Sequence similarities

      Belongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • 15 lipoxygenase 1 antibody
        • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
        • 15 LOX antibody
        • 15 LOX 1 antibody
        • 15-LOX antibody
        • 15LOX 1 antibody
        • ALOX15 antibody
        • Arachidonate 15 lipoxygenase antibody
        • Arachidonate 15-lipoxygenase antibody
        • Arachidonate omega 6 lipoxygenase antibody
        • Arachidonate omega-6 lipoxygenase antibody
        • LOG15 antibody
        • LOX15_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

        Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in eosinophils of mouse colon (PMID:23503644).

        The nuclear counter stain is DAPI (blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

        Lane 1 : Mouse lung lysate
        Lane 2 : Rat lung lysate
        Lane 3 : Human colon lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 75 kDa
        Observed band size: 75 kDa



        Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 15 seconds.

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

      • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

        Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
        Lane 2 : HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 75 kDa
        Observed band size: 75 kDa


        Exposure time: 3 seconds


        Blocking/Dilution buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

        Immunohistochemical analysis of paraffin-embedded rat bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of rat bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

        Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of mouse colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

        Immunohistochemical analysis of paraffin-embedded human bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of human bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

        Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      • Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
        Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab244205? Please let us know so that we can cite the reference in this datasheet.

      ab244205 has not yet been referenced specifically in any publications.

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