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    15-lipoxygenase-1-antibody-epr22138-ab244205.pdf

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RecombinantRabMAb

Recombinant Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

  • Datasheet
  • Certificate of Compliance
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Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
  • Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human 15 Lipoxygenase 1 protein (ab114421)
Biochemical
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Oil Red O, Lysochrome diazo dye (ab146295)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-15 Lipoxygenase 1 antibody [EPR22138]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22138] to 15 Lipoxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate; Mouse and rat lung lysates; Human colon lysate. IHC-P: Human colon and bronchus tissues; Mouse colon tissue; Rat bronchus tissue. IHC-Fr: Mouse colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22138
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-15 Lipoxygenase 1 antibody [EPR22138] - BSA and Azide free (ab245201)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Recombinant Protein

    • Recombinant Human 15 Lipoxygenase 1 protein (ab114421)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr
1/500.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr
1/500.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway

    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P16050 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 246 Human
    • Entrez Gene: 11687 Mouse
    • Entrez Gene: 81639 Rat
    • Omim: 152392 Human
    • SwissProt: P16050 Human
    • SwissProt: P39654 Mouse
    • SwissProt: Q02759 Rat
    • Unigene: 73809 Human
    • Alternative names

      • 15 lipoxygenase 1 antibody
      • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
      • 15 LOX antibody
      • 15 LOX 1 antibody
      • 15-LOX antibody
      • 15LOX 1 antibody
      • ALOX15 antibody
      • Arachidonate 15 lipoxygenase antibody
      • Arachidonate 15-lipoxygenase antibody
      • Arachidonate omega 6 lipoxygenase antibody
      • Arachidonate omega-6 lipoxygenase antibody
      • LOG15 antibody
      • LOX15_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in eosinophils of mouse colon (PMID:23503644).

      The nuclear counter stain is DAPI (blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate
      Lane 3 : Human colon lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa



      Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 15 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Western blot - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205) at 1/1000 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293 transfected with 15 Lipoxygenase 1 (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30118527; PMID:1662607).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Immunohistochemical analysis of paraffin-embedded rat bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of rat bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of mouse colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Immunohistochemical analysis of paraffin-embedded human bronchus tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelial cells of human bronchus (PMID:24036245; PMID:21831839). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling 15 Lipoxygenase 1 with ab244205 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in eosinophils of human colon (PMID:30118527; PMID:23503644). Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)
      Anti-15 Lipoxygenase 1 antibody [EPR22138] (ab244205)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab244205? Please let us know so that we can cite the reference in this datasheet.

    ab244205 has been referenced in 1 publication.

    • Tian Q  et al. Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma. Front Cell Dev Biol 9:684259 (2021). PubMed: 34307361

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-15 Lipoxygenase 1 antibody [EPR22138]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (macrophage)
    Permeabilization
    Yes - 0.1%triton x100
    Specification
    macrophage
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    DR. Prashant Rai

    Verified customer

    Submitted Aug 31 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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