Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

Abreviews (1)Q&A (2)Specific References (6)

Overview

  • Product name
    Anti-15 Lipoxygenase 1 antibody [OTI3G8]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description
    Mouse monoclonal [OTI3G8] to 15 Lipoxygenase 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length, Human 15 Lipoxygenase 1 produced in HEK293T cells (NP_001131).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA; HepG2, HeLa, A549, COS7, MDCK, PC12 and MCF7 cell extracts; Human breast, Human pancreas carcinoma, Human thyroid carcinoma, Human endometrium, Human endometrium adenocarcinoma, Human prostate carcinoma and Human bladder carcinoma; COS7 cells transiently transfected by pCMV6-ENTRY 15 Lipoxygenase 1.
  • General notes

    The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab119774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 75 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • Function
    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway
    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 15 lipoxygenase 1 antibody
      • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
      • 15 LOX antibody
      • 15 LOX 1 antibody
      • 15-LOX antibody
      • 15LOX 1 antibody
      • ALOX15 antibody
      • Arachidonate 15 lipoxygenase antibody
      • Arachidonate 15-lipoxygenase antibody
      • Arachidonate omega 6 lipoxygenase antibody
      • Arachidonate omega-6 lipoxygenase antibody
      • LOG15 antibody
      • LOX15_HUMAN antibody
      see all

    Images

    • Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

      Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
      Lane 2 : HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 75 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209418
    • Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

      Lane 1 : HepG2 cell extracts
      Lane 2 : HeLa cell extracts
      Lane 3 : SVT2 cell extracts
      Lane 4 : A549 cell extracts
      Lane 5 : COS7 cell extracts
      Lane 6 : Jurkat cell extracts
      Lane 7 : MDCK cell extracts
      Lane 8 : PC12 cell extracts
      Lane 9 : MCF7 cell extracts

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 75 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209486
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human breast tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
      ab119774 at 1/100, staining 15 Lipoxygenase 1 in COS7 cells transiently transfected with pCMV6-ENTRY 15 Lipoxygenase 1, by immunofluorescence.

    References

    This product has been referenced in:
    • Werz O  et al. Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity. Nat Commun 9:59 (2018). WB, IF ; Human . Read more (PubMed: 29302056) »
    • Ho CF  et al. Expression of DHA-Metabolizing Enzyme Alox15 is Regulated by Selective Histone Acetylation in Neuroblastoma Cells. Neurochem Res 43:540-555 (2018). WB, ICC/IF ; Human . Read more (PubMed: 29235036) »

    See all 6 Publications for this product

    Publishing research using ab119774? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

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    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-15 Lipoxygenase 1 antibody [3G8]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (prostate cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6, 100C, 20 min
    Permeabilization
    No
    Specification
    prostate cancer
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
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    Abcam user community

    Verified customer

    Submitted May 05 2015

    Please see below the responses to your questions. Hopefully these will help you fix the problems. However, I want to convey you that I dont need a replacement as the purpose was solved by reverting to antibodies from other sources. I can provide you th...

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    Thank you very much for sending all of this information.

    I'm sending free of charge vials of ab39626 and ab39628 on the order ***, which should arrive on Monday.

    Just to confirm, the macrophages were from mice or a different species...

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    As evident from my account history, I have extensively used your product and had minimal problems with the antibodies.

    However, two of the antibodies from my last order never worked. I ended up in ordering antibodies from other sources and h...

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    Thank you so much for contacting us and for letting us know about the trouble with these two antibodies.

    We do fully guarantee our products to work as stated on the datasheets in tested species and applications, so if the antibodies did not w...

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