Name: Anti-15 Lipoxygenase 1 antibody [OTI3G8]
SKU: ab119774
Rating: 4 (1 reviews)

Product name

Anti-15 Lipoxygenase 1 antibody [OTI3G8]
See all 15 Lipoxygenase 1 primary antibodies
Description

Mouse monoclonal [OTI3G8] to 15 Lipoxygenase 1
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Rat, Dog, Human, Monkey
Immunogen

Recombinant full length, Human 15 Lipoxygenase 1 produced in HEK293T cells (NP_001131).
Positive control
- HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA; HepG2, HeLa, A549, COS7, MDCK, PC12 and MCF7 cell extracts; Human breast, Human pancreas carcinoma, Human thyroid carcinoma, Human endometrium, Human endometrium adenocarcinoma, Human prostate carcinoma and Human bladder carcinoma; COS7 cells transiently transfected by pCMV6-ENTRY 15 Lipoxygenase 1.
General notes

The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

OTI3G8
Isotype

IgG2b
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Positive Controls

Our Abpromise guarantee covers the use of ab119774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/2000. Predicted molecular weight: 75 kDa.
IHC-P		1/150.
ICC/IF		1/100.

Function

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
Pathway

Lipid metabolism; leukotriene D4 biosynthesis.
Sequence similarities

Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P16050 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 246 Human
- Entrez Gene: 81639 Rat
- Omim: 152392 Human
- SwissProt: P16050 Human
- SwissProt: Q02759 Rat
- Unigene: 73809 Human
Alternative names
- 15 lipoxygenase 1 antibody
- 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
- 15 LOX antibody
- 15 LOX 1 antibody
- 15-LOX antibody
- 15LOX 1 antibody
- ALOX15 antibody
- Arachidonate 15 lipoxygenase antibody
- Arachidonate 15-lipoxygenase antibody
- Arachidonate omega 6 lipoxygenase antibody
- Arachidonate omega-6 lipoxygenase antibody
- LOG15 antibody
- LOX15_HUMAN antibody
see all

Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 75 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209418
Western blot - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

Lane 1 : HepG2 cell extracts
Lane 2 : HeLa cell extracts
Lane 3 : SVT2 cell extracts
Lane 4 : A549 cell extracts
Lane 5 : COS7 cell extracts
Lane 6 : Jurkat cell extracts
Lane 7 : MDCK cell extracts
Lane 8 : PC12 cell extracts
Lane 9 : MCF7 cell extracts

Lysates/proteins at 35 µg per lane.

Predicted band size: 75 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209486
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human breast tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

ab119774 at 1/100, staining 15 Lipoxygenase 1 in COS7 cells transiently transfected with pCMV6-ENTRY 15 Lipoxygenase 1, by immunofluorescence.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Werz O et al. Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity. Nat Commun 9:59 (2018). WB, IF ; Human . Read more (PubMed: 29302056) »
Ho CF et al. Expression of DHA-Metabolizing Enzyme Alox15 is Regulated by Selective Histone Acetylation in Neuroblastoma Cells. Neurochem Res 43:540-555 (2018). WB, ICC/IF ; Human . Read more (PubMed: 29235036) »

See all 7 Publications for this product

Publishing research using ab119774? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (prostate cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate, pH6, 100C, 20 min

Permeabilization

Specification

prostate cancer

Blocking step

H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted May 05 2015

Question

Please see below the responses to your questions. Hopefully these will help you fix the problems. However, I want to convey you that I dont need a replacement as the purpose was solved by reverting to antibodies from other sources. I can provide you the info. that would tell that I had indeed bought these anitbodies from other sources. However, I would greatly appreciate if you could send me the other two anitbodies that I have been using from you and are wuite robust and working great. This will help me save some money.

1) What application were the antibodies used in? What kind of results were seen (high background, no staining/bands, etc)?

Mouse monoclonal [3G8] to 15 Lipoxygenase 1: Used for western on macrophages protein extracts. 1:500. No band was seen. After frustrations, the western data was generated using other ALOX15 antibody that worked really well.

Rabbit polyclonal to CD3: used for Immunohistochemstry (fluorescent) on frozen spleen. (1:100 conc. was used. O/N in cold room) Staining was not achieved. Again, tried other antibody that worked and the data was generated.

2) What species were the samples from? What kind of tissue/cells were used?

Mouse monoclonal [3G8] to 15 Lipoxygenase 1: mouse alveolar as well as BMDM macrophages.

Rabbit polyclonal to CD3: mouse frozen tissue sections, spleen.

3) Could you describe the protocol used? Please include the antibody dilution/concentration and components of the blocking solution.

Blocking for western was odyssey blocking buffer by Li-Cor.

For IHC, blocked was 5% goat serum in PBST.

Thanks

Abcam community

Verified customer

Asked on Jul 13 2012

Answer

Thank you very much for sending all of this information.

I'm sending free of charge vials of ab39626 and ab39628 on the order ***, which should arrive on Monday.

Just to confirm, the macrophages were from mice or a different species? We haven't tested ab119774 with mouse samples, so wecan't guarantee that it reacts with the mouse protein. If you'd ever like to try an antibody with an untested species, we do have a testing discount program that you might be interested in. Feel free to contact me if you'd ever like more information.

If you have any questions or if there is anything else that we can do for you, please let me know and I'll be happy to help. Have a nice weekend.

Abcam Scientific Support

Answered on Jul 13 2012

Question

As evident from my account history, I have extensively used your product and had minimal problems with the antibodies.

However, two of the antibodies from my last order never worked. I ended up in ordering antibodies from other sources and have used them to generate the data.

Since I have wasted lot of time and resources on getting these two antibodies worked, I thought I should request either refund or replacement for 15-ALOX and CD3 antibodies.

I am very happy with FIZZ1 antibodies (AB39626) and AB39628. I would appreciate if you could send me these two antibodies or issue me refund/credit.

Sorry to bother you.

Thanks

Abcam community

Verified customer

Asked on Jul 12 2012

Answer

Thank you so much for contacting us and for letting us know about the trouble with these two antibodies.

We do fully guarantee our products to work as stated on the datasheets in tested species and applications, so if the antibodies did not work as they should have, we can provide replacements. For our records, could you please tell me the following information?

1) What application were the antibodies used in? What kind of results were seen (high background, no staining/bands, etc)?

2) What species were the samples from? What kind of tissue/cells were used?

3) Could you describe the protocol used? Please include the antibody dilution/concentration and components of the blocking solution.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Abcam Scientific Support

Answered on Jul 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

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This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-15 Lipoxygenase 1 antibody [3G8]