Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)
Key features and details
- Mouse monoclonal [OTI3G8] to 15 Lipoxygenase 1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-15 Lipoxygenase 1 antibody [OTI3G8]
See all 15 Lipoxygenase 1 primary antibodies -
Description
Mouse monoclonal [OTI3G8] to 15 Lipoxygenase 1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length, Human 15 Lipoxygenase 1 produced in HEK293T cells (NP_001131).
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Positive control
- HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA; HepG2, HeLa, A549, COS7, MDCK, PC12 and MCF7 cell extracts; Human breast, Human pancreas carcinoma, Human thyroid carcinoma, Human endometrium, Human endometrium adenocarcinoma, Human prostate carcinoma and Human bladder carcinoma; COS7 cells transiently transfected by pCMV6-ENTRY 15 Lipoxygenase 1.
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General notes
The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI3G8 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/150.
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ICC/IF |
1/100.
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Notes |
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IHC-P
1/150. |
ICC/IF
1/100. |
Target
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Function
Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. -
Pathway
Lipid metabolism; leukotriene D4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 246 Human
- Omim: 152392 Human
- SwissProt: P16050 Human
- Unigene: 73809 Human
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Alternative names
- 15 lipoxygenase 1 antibody
- 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
- 15 LOX antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human breast tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
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ab119774 at 1/100, staining 15 Lipoxygenase 1 in COS7 cells transiently transfected with pCMV6-ENTRY 15 Lipoxygenase 1, by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab119774 has been referenced in 15 publications.
- Sun X et al. The modification of ferroptosis and abnormal lipometabolism through overexpression and knockdown of potential prognostic biomarker perilipin2 in gastric carcinoma. Gastric Cancer 23:241-259 (2020). PubMed: 31520166
- Rao Z et al. Vacuolar (H+)-ATPase Critically Regulates Specialized Proresolving Mediator Pathways in Human M2-like Monocyte-Derived Macrophages and Has a Crucial Role in Resolution of Inflammation. J Immunol 203:1031-1043 (2019). PubMed: 31300512
- Dakin SG et al. Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears. Am J Pathol 189:2258-2268 (2019). PubMed: 31437425
- Werner M et al. Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome. FASEB J 33:6140-6153 (2019). PubMed: 30735438
- Dakin SG et al. 15-Epi-LXA4 and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture. FASEB J N/A:fj201900196R (2019). PubMed: 30916999