Overview

  • Product name

  • Description

    Rabbit polyclonal to 15-LOX-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rabbit, Pig
  • Immunogen

    Synthetic peptide corresponding to Human 15-LOX-2 aa 163-181.
    Sequence:

    CLDEKTVEDLELNIKYSTA

  • Positive control

    • Human prostate homogenate.

Properties

Applications

Our Abpromise guarantee covers the use of ab23691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ICC/IF
WB
ICC
  • Application notes
    ICC: Use at a concentration of 6 µg/ml or 1:100
    WB: Use at a concentration of 3 µg/ml or 1:200. Predicted molecular weight: 76 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized.
    • Tissue specificity

      Expressed in hair, prostate, lung and cornea.
    • Pathway

      Lipid metabolism; leukotriene D4 biosynthesis.
    • Sequence similarities

      Belongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 15 Lipoxygenase 2 antibody
      • 15 LO 2 antibody
      • 15 LOX 2 antibody
      • 15-lipoxygenase 2 antibody
      • 15-LOX-2 antibody
      • 15-LOX-B antibody
      • Alox15b antibody
      • Arachidonate 15 lipoxygenase second type antibody
      • Arachidonate 15 lipoxygenase type II antibody
      • Arachidonate 15-lipoxygenase B antibody
      • Arachidonate 15-lipoxygenase type II antibody
      • LX15B_HUMAN antibody
      see all

    Images

    • Immunofluorescence analysis of murine embryonic fibroblasts, staining 15-LOX-2 with ab23691.

      Cells were fixed with paraformaldehyde, permeabilized with 0.2 % Triton-X-100 and blocked with 2% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in 1% BSA in TBST) for 16 hours at 4°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-15-LOX-2 antibody (ab23691) at 4 µg/ml

      Lane 1 : Rabbit reticulocyte lysate (30 µg).
      Lane 2 : Human prostate homogenate (30 µg).

      Predicted band size: 76 kDa
      Observed band size: 76 kDa

    • ab23691 staining 15-LOX-2 in human artery tissue sections by IHC-Fr (frozen sections). Tissue samples were fixed with acetone and blocked with 0.5% BSA for 10 minutes.  Samples were incubated with primary antibody 1/100  24 hours.  A  biotin-conjugated horse anti Rabbit IgG (H+L) polyclonal  (7 µg/ml) was used as secondary antibody.

      See Abreview

    References

    This product has been referenced in:

    • Sandstedt M  et al. Hypoxic cardiac fibroblasts from failing human hearts decrease cardiomyocyte beating frequency in an ALOX15 dependent manner. PLoS One 13:e0202693 (2018). Read more (PubMed: 30138423) »
    • Rodríguez-Blanco G  et al. Tissue proteomics outlines AGR2 AND LOX5 as markers for biochemical recurrence of prostate cancer. Oncotarget 9:36444-36456 (2018). Read more (PubMed: 30559929) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (murine embryonic fibroblasts)
    Permeabilization
    Yes - 0.2 % Triton-X-100
    Specification
    murine embryonic fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 20 2012

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (artery)
    Permeabilization
    No
    Specification
    artery
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 0.5%
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Nov 24 2009

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2-OS)
    Permeabilization
    Yes - 0.1% Triton-X-100/PBS
    Specification
    U2-OS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 25 2008

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