Overview

  • Product name

    Anti-15-PGDH antibody [EPR14332-19]
    See all 15-PGDH primary antibodies
  • Description

    Rabbit monoclonal [EPR14332-19] to 15-PGDH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human 15-PGDH aa 50-200. The exact sequence is proprietary.
    Database link: P15428

  • Positive control

    • SW480, LoVo, Mouse small intestine and Rat small intestine lysates; SW480 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.
  • Tissue specificity

    Detected in colon epithelium (at protein level).
  • Involvement in disease

    Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) [MIM:259100]; also known as pachydermoperiostosis autosomal recessive. Primary hypertrophic osteoarthropathy is characterized by digital clubbing, osterarthropathy, variable features of pachydermia, delayed closure of the fontanels, and congenital heart disease.
    Defects in HPGD are the cause of cranioosteoarthropathy (COA) [MIM:259100]. Clinical features include infantile onset of swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature.
    Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC) [MIM:119900]; also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 15 hydroxyprostaglandin dehydrogenase [NAD+] antibody
    • 15 PGDH antibody
    • 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody
    • 15-PGDH antibody
    • 15PGDH antibody
    • Hpgd antibody
    • Hydroxyprostaglandin dehydrogenase 15 (NAD) antibody
    • NAD+ dependent 15 hydroxyprostaglandin dehydrogenase antibody
    • OTTHUMP00000218960 antibody
    • OTTHUMP00000219016 antibody
    • OTTHUMP00000219018 antibody
    • PGDH antibody
    • PGDH_HUMAN antibody
    • PGDH1 antibody
    • PHOAR1 antibody
    • Prostaglandin dehydrogenase 1 antibody
    • SDR36C1 antibody
    • Short chain dehydrogenase/reductase family 36C member 1 antibody
    see all

Images

  • All lanes : Anti-15-PGDH antibody [EPR14332-19] (ab187161) at 1/1000 dilution

    Lane 1 : SW480 lysate
    Lane 2 : LoVo lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-15-PGDH antibody [EPR14332-19] (ab187161) at 1/1000 dilution

    Lane 1 : Mouse small intestine lysate
    Lane 2 : Rat small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Immunofluorescent analysis of SW480 cells labeling 15-PGDH with ab187161 at 1/250 and DAPI staining (blue). An anti rabbit IgG (Alexa Fluor®555) was used as the secondary.

References

This product has been referenced in:

  • Arima K  et al. Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1ß from activated macrophages leads to poor prognosis in pancreatic cancer. Cancer Sci 109:462-470 (2018). Read more (PubMed: 29224225) »
See 1 Publication for this product

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