1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)Picture courtesy of Dr. Gerry Shaw, University of Florida
Immunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a
red fluorescence. Blue nuclei demonstrate DAPI DNA staining.