Overview

  • Product name
    Anti-160 kD Neurofilament Medium antibody
    See all 160 kD Neurofilament Medium primary antibodies
  • Description
    Chicken polyclonal to 160 kD Neurofilament Medium
  • Host species
    Chicken
  • Tested applications
    Suitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant NFM protein expressed in bacteria followed by hens co-boosted with recombinant NFM protein expressed in bacteria

  • Positive control
    • Dissociated cell culture prepared from an adult rat brain.
  • General notes
    Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

Properties

Applications

Our Abpromise guarantee covers the use of ab134458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/2000 - 1/5000. Use 2% paraformaldehyde-fixed tissues.
WB 1/5000 - 1/10000. Predicted molecular weight: 98 kDa.
ICC/IF 1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.

Target

  • Relevance
    Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localization
    Cytoplasm
  • Database links
  • Alternative names
    • 150kDa medium antibody
    • NEF3 antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • NF160 antibody
    • NFM antibody
    see all

Images

  • Immunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.

References

ab134458 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134458.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up