Anti-160 kD Neurofilament Medium antibody (ab134458)
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- References
- Protocols
Overview
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Product name
Anti-160 kD Neurofilament Medium antibody
See all 160 kD Neurofilament Medium primary antibodies -
Description
Chicken polyclonal to 160 kD Neurofilament Medium -
Host species
Chicken -
Tested applications
Suitable for: IHC-FoFr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant NFM protein expressed in bacteria followed by hens co-boosted with recombinant NFM protein expressed in bacteria
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Positive control
- Dissociated cell culture prepared from an adult rat brain.
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General notes
Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer and then filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/2000 - 1/5000. Use 2% paraformaldehyde-fixed tissues. | |
| WB | 1/5000 - 1/10000. Predicted molecular weight: 98 kDa. | |
| ICC/IF | 1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
Target
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Relevance
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. -
Cellular localization
Cytoplasm -
Database links
- Entrez Gene: 4741 Human
- Entrez Gene: 18040 Mouse
- Entrez Gene: 24588 Rat
- Omim: 162250 Human
- SwissProt: P07197 Human
- SwissProt: P08553 Mouse
- SwissProt: P12839 Rat
- Unigene: 458657 Human
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Alternative names
- 150kDa medium antibody
- NEF3 antibody
- NEFM antibody
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Images
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Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)Picture courtesy of Dr. Gerry Shaw, University of FloridaImmunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.
Protocols
Datasheets and documents
References
ab134458 has not yet been referenced specifically in any publications.
