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Anti-160 kD Neurofilament Medium antibody (ab134458)

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Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)

    Key features and details

    • Chicken polyclonal to 160 kD Neurofilament Medium
    • Suitable for: ICC/IF
    • Reacts with: Rat
    • Isotype: IgY

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    Overview

    • Product name

      Anti-160 kD Neurofilament Medium antibody
      See all 160 kD Neurofilament Medium primary antibodies
    • Description

      Chicken polyclonal to 160 kD Neurofilament Medium
    • Host species

      Chicken
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Recombinant NFM protein expressed in bacteria followed by hens co-boosted with recombinant NFM protein expressed in bacteria

    • General notes

      Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.02% Sodium azide
      Constituents: 98% PBS, 1% BSA
    • Concentration information loading...
    • Purity

      IgY fraction
    • Purification notes

      Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer and then filter-sterilized.
    • Clonality

      Polyclonal
    • Isotype

      IgY
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Neuroscience
      • Cell Adhesion Proteins
      • Cytoskeletal Proteins
      • Intermediate Filaments
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Soma marker
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class IV
      • Neurofilaments
      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Developmental Biology
      • Lineage specification
      • Ectoderm

    Associated products

    • Compatible Secondaries

      • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
      • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
      • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
      • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
      • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
    • Isotype control

      • Chicken IgY - Isotype Control (ab50579)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab134458 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
    Notes
    ICC/IF
    1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.

    Target

    • Relevance

      Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
    • Cellular localization

      Cytoplasm
    • Database links

      • Entrez Gene: 24588 Rat
      • SwissProt: P12839 Rat
      • Alternative names

        • 150kDa medium antibody
        • NEF3 antibody
        • NEFM antibody
        • Neurofilament 3 antibody
        • NF160 antibody
        • NFM antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)
        Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)Picture courtesy of Dr. Gerry Shaw, University of Florida
        Immunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab134458? Please let us know so that we can cite the reference in this datasheet.

      ab134458 has been referenced in 2 publications.

      • Lian JJ  et al. Flumazenil-Insensitive Benzodiazepine Effects in Recombinant aß and Neuronal GABAA Receptors. Brain Sci 10:N/A (2020). PubMed: 32150806
      • DiCello JJ  et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144

      Customer reviews and Q&As

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