Key features and details
- Chicken polyclonal to 160 kD Neurofilament Medium
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgY
Product nameAnti-160 kD Neurofilament Medium antibody
See all 160 kD Neurofilament Medium primary antibodies
DescriptionChicken polyclonal to 160 kD Neurofilament Medium
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Recombinant fusion protein containing the extreme C-terminus of rat 160 kD Neurofilament Medium protein expressed in and purified from E. coli.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab72998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 145 kDa (predicted molecular weight: 96 kDa).|
RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
- 150kDa medium antibody
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Anti-160 kD Neurofilament Medium antibody (ab72998) at 1/5000 dilution + rat cerebral cortex lysate
Predicted band size: 96 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
ab72998 has been referenced in 2 publications.
- Decourtye L et al. Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation. PLoS One 13:e0193196 (2018). PubMed: 29466413
- Decourtye L et al. IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice. PLoS One 12:e0170083 (2017). PubMed: 28076448