Key features and details
- Rabbit polyclonal to 160 kD Neurofilament Medium
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat, Pig
- Isotype: IgG
Product nameAnti-160 kD Neurofilament Medium antibody
See all 160 kD Neurofilament Medium primary antibodies
DescriptionRabbit polyclonal to 160 kD Neurofilament Medium
SpecificityRecognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa).
Tested applicationsSuitable for: IHC-FrFl, WBmore details
Species reactivityReacts with: Mouse, Rat, Pig
Predicted to work with: Cat, Bird, Reptile
Recombinant fragment corresponding to Rat 160 kD Neurofilament Medium (C terminal).
Database link: P12839
- IHC-FrFl: Rat cerebellum WB: Mouse, Rat and Pig brain and spinal cord tissue lysates
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Our Abpromise guarantee covers the use of ab9034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
- Information by UniProt
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
Immunohistochemistry - Free Floating analysis of rat cerebellum section labeling 160 kD Neurofilament Medium with ab9034 at 1/2000 diution (red) and and costained with mouse mAb to GAP43 at 1/2000 diution (green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab9034 strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
All lanes : Anti-160 kD Neurofilament Medium antibody (ab9034) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Pig spinal cord tissue lysate
ab9034 has been referenced in 14 publications.
- Mazzone SB et al. Transcriptional Profiling of Individual Airway Projecting Vagal Sensory Neurons. Mol Neurobiol 57:949-963 (2020). PubMed: 31630330
- Ádám Á et al. Gestational Exposure to Sodium Valproate Disrupts Fasciculation of the Mesotelencephalic Dopaminergic Tract, With a Selective Reduction of Dopaminergic Output From the Ventral Tegmental Area. Front Neuroanat 14:29 (2020). PubMed: 32581730
- Morad T et al. Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis. Polymers (Basel) 12:N/A (2020). PubMed: 33260420
- Shah S et al. Nitrosative Stress Is Associated with Dopaminergic Dysfunction in the HIV-1 Transgenic Rat. Am J Pathol 189:1375-1385 (2019). PubMed: 31230667
- Driessen AK et al. Reflex regulation of breathing by the paratrigeminal nucleus via multiple bulbar circuits. Brain Struct Funct 223:4005-4022 (2018). PubMed: 30116890