Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y]
See all 160 kD Neurofilament Medium primary antibodies
DescriptionRabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human 160 kD Neurofilament Medium
- SH-SY5Y cell lysates; human brain tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
- 150kDa medium antibody
- NEF3 antibody
- NEFM antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution
Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
Lane 2 : Untreated SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
ab68142 has not yet been referenced specifically in any publications.