Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Product nameAnti-17-beta-Estradiol antibody [EPPTX-R2]
See all 17-beta-Estradiol primary antibodies
DescriptionRabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol
Tested applicationsSuitable for: Competitive ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Human 17-beta-Estradiol. Immunogen is Estradiol-6-CMO-BSA.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.
Use 3-5 ng/ml.
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
ab215528 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.
The data was kindly provided by Pantex Division of Bio-Analysis, Inc.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215528 has not yet been referenced specifically in any publications.