Recombinant
RabMAb

Recombinant Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (ab224259)

Overview

  • Product name

    Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free
    See all 17-beta-Estradiol primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-R2] to 17-beta-Estradiol - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule. Immunogen is Estradiol-6-CMO-BSA.

  • General notes

    Ab224259 is the carrier-free version of ab215528. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPPTX-R2
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab224259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • ab224259 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.

    The data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • This ELISA data used the same anti-17-beta-Estradiol antibody clone [EPPTX-R2] in a different buffer formulation (cat# ab215528).

    Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

References

ab224259 has not yet been referenced specifically in any publications.

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