17 beta Estradiol ELISA Kit (ab108667)
- Datasheet
- References (10)
- Protocols
Overview
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Product name17 beta Estradiol ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 20 <=9% Inter-assay Sample n Mean SD CV% Overall 3 <=10% -
Sample typeUrine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
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Assay typeCompetitive
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Sensitivity8.68 pg/ml
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Range20 pg/ml - 2000 pg/ml
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Recovery
101.09 %
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Mammals
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Product overview
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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Tested applicationsSuitable for: Competitive ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Washing Solution 1 x 50ml 17 beta Estradiol Control 1 x 0.5ml 17 beta Estradiol Standard 0 1 x 1ml 17 beta Estradiol Standard 1 (20 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 2 (120 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 3 (300 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 4 (600 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 5 (2,000 pg/mL) 1 x 0.5ml 17 beta Estradiol-HRP Conjugate 1 x 22ml Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab108667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent dilution. |
Images
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Biological fluids for ab10866717 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108667
Protocols
References
This product has been referenced in:
- Wickham KA et al. No effect of beetroot juice supplementation on exercise economy and performance in recreationally active females despite increased torque production. Physiol Rep 7:e13982 (2019). Read more (PubMed: 30653856) »
- Weitzmann MN et al. Reduced bone formation in males and increased bone resorption in females drive bone loss in hemophilia A mice. Blood Adv 3:288-300 (2019). Read more (PubMed: 30700417) »
