17 beta Estradiol ELISA Kit (ab108667)
Overview
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Product name
17 beta Estradiol ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 20 <=9% Inter-assay Sample n Mean SD CV% Overall 3 <=10% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
8.68 pg/ml -
Range
20 pg/ml - 2000 pg/ml -
Recovery
101.09 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Mammals
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Product overview
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Washing Solution 1 x 50ml 17 beta Estradiol Control 1 x 0.5ml 17 beta Estradiol Standard 0 1 x 1ml 17 beta Estradiol Standard 1 (20 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 2 (120 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 3 (300 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 4 (600 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 5 (2,000 pg/mL) 1 x 0.5ml 17 beta Estradiol-HRP Conjugate 1 x 22ml Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Biological fluids for ab10866717 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108667
References
ab108667 has been referenced in 23 publications.
- Skórkowska A et al. Effect of Combined Prenatal and Adult Benzophenone-3 Dermal Exposure on Factors Regulating Neurodegenerative Processes, Blood Hormone Levels, and Hematological Parameters in Female Rats. Neurotox Res 37:683-701 (2020). PubMed: 31970650
- Ben Hameid AS et al. Substantial Effect of Fenugreek Seeds Aqueous Extract on Serum Estradiol Level in Ovarian Hyperstimulation Syndrome Rat Model. Oman Med J 34:238-243 (2019). PubMed: 31110632
- Wickham KA et al. No effect of beetroot juice supplementation on exercise economy and performance in recreationally active females despite increased torque production. Physiol Rep 7:e13982 (2019). PubMed: 30653856
- Weitzmann MN et al. Reduced bone formation in males and increased bone resorption in females drive bone loss in hemophilia A mice. Blood Adv 3:288-300 (2019). PubMed: 30700417
- Delgobo M et al. N-acetylcysteine and alpha-lipoic acid improve antioxidant defenses and decrease oxidative stress, inflammation and serum lipid levels in ovariectomized rats via estrogen-independent mechanisms. J Nutr Biochem 67:190-200 (2019). PubMed: 30951973
