Overview

  • Product name
    17 beta Estradiol ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 20 <=9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 <=10%
  • Sample type
    Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Competitive
  • Sensitivity
    8.68 pg/ml
  • Range
    20 pg/ml - 2000 pg/ml
  • Recovery

    101.09 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammals
  • Product overview

    Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).

    A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • 17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.

  • Representative Standard Curve using ab108667

Protocols

References

This product has been referenced in:
  • Wickham KA  et al. No effect of beetroot juice supplementation on exercise economy and performance in recreationally active females despite increased torque production. Physiol Rep 7:e13982 (2019). Read more (PubMed: 30653856) »
  • Weitzmann MN  et al. Reduced bone formation in males and increased bone resorption in females drive bone loss in hemophilia A mice. Blood Adv 3:288-300 (2019). Read more (PubMed: 30700417) »
See all 15 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

It is recommended to use the kit component 'Estradiol 17-beta Standard 0' to dilute high value samples. The standard is prepared from 17 Beta-Estradiol free serum. Alternatively, serum from males free of Estradiol should be use as the base to dilute high samples. The use of PBS/1% BSA does not mimic the matrix or serum samples and could result in unreliable results.

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Answer

Our 17 Beta-Estradiol ELISA ab08667 is a competitive immunoassay. Therefore, one would expect an inverse relationship between the absorbance and concentration.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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