Question (72649) | 17 beta Estradiol ELISA Kit (ab108667)

Go to datasheet (ab108667)

Question

I have serum that gives to high of a signal at full strength. I typically dilute in PBS/1% BSA. Is this dilution buffer compatible with this kit?

Answer

It is recommended to use the kit component 'Estradiol 17-beta Standard 0' to dilute high value samples. The standard is prepared from 17 Beta-Estradiol free serum. Alternatively, serum from males free of Estradiol should be use as the base to dilute high samples. The use of PBS/1% BSA does not mimic the matrix or serum samples and could result in unreliable results.

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