Overview

  • Product name

    17 OH Progesterone ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Control Sera 3 <=8.2%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 3 <=13.8%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.05 ng/ml
  • Recovery

    98.66 %

  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.

     

    A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108668

Protocols

References

This product has been referenced in:

  • Fu Q  et al. Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27136540) »
See 1 Publication for this product

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