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Recombinant full length protein (Human).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab16765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 24 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
ICC/IF image of ab16765 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16765, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Western blot analysis using ab16765 at 1/1000 dilution using HeLa cell lysates:
Lane 1: Recombinant human PrxI proetein
Lane 2: Recombinant human PrxII protein
Lane 3: Recombinant human PrxIII protein
Lane 4: Recombinant human PrxIV protein (w/o secretion leader sequence)
Lane 5: HeLa cell lysates.Western blot analysis using ab16765 at 1/1000 dilution using HeLa cell lysates: Lane 1: Recombinant human PrxI proetein Lane 2: Recombinant human PrxII protein Lane 3: Recombinant human PrxIII protein Lane 4: Recombinant human PrxIV protein (w/o secretion leader sequence) Lane 5: HeLa cell lysates.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"