Key features and details
- Rabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody
See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies
DescriptionRabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human 2 Hydroxy phytanoyl CoA lyase/HACL1 aa 528-578. The exact sequence is proprietary.
Database link: Q9UJ83
- WB: HeLa, HEK-293T, Jurkat, NIH/3T3 and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to 2 Hydroxy phytanoyl CoA lyase/HACL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
FunctionCatalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
Tissue specificityExpressed in high levels in liver, also expressed in kidney, heart and skeletal muscle.
PathwayLipid metabolism; fatty acid metabolism.
Sequence similaritiesBelongs to the TPP enzyme family.
- Information by UniProt
- 1600020H07Rik antibody
- 2 HPCL antibody
- 2 hydroxyacyl CoA lyase 1 antibody
All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241353 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.
Lane 1: ab241353 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241353 has not yet been referenced specifically in any publications.