Overview

  • Product name

    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody
    See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies
  • Description

    Rabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human 2 Hydroxy phytanoyl CoA lyase/HACL1 aa 528-578. The exact sequence is proprietary.
    Database link: Q9UJ83

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, NIH/3T3 and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab241353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent concentration.

20 µl/mg lysate

Target

  • Function

    Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
  • Tissue specificity

    Expressed in high levels in liver, also expressed in kidney, heart and skeletal muscle.
  • Pathway

    Lipid metabolism; fatty acid metabolism.
  • Sequence similarities

    Belongs to the TPP enzyme family.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1600020H07Rik antibody
    • 2 HPCL antibody
    • 2 hydroxyacyl CoA lyase 1 antibody
    • 2 hydroxyphytanoyl CoA lyase antibody
    • 2-HPCL antibody
    • 2-hydroxyacyl-CoA lyase 1 antibody
    • 2-hydroxyphytanoyl-CoA lyase antibody
    • FLJ55041 antibody
    • Hacl1 antibody
    • HACL1_HUMAN antibody
    • HPCL antibody
    • HPCL2 antibody
    • HSPC279 antibody
    • PHYH2 antibody
    • Phytanoyl CoA 2 hydroxylase 2 antibody
    • Phytanoyl CoA hydroxylase 2 antibody
    • Phytanoyl-CoA 2-hydroxylase 2 antibody
    see all

Images

  • All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • 2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab241353 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.

    Lane 1: ab241353 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Chemiluminescence detection: 30 seconds.

References

ab241353 has not yet been referenced specifically in any publications.

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