Product nameAnti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody
See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies
DescriptionRabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human 2 Hydroxy phytanoyl CoA lyase/HACL1 aa 528-578. The exact sequence is proprietary.
Database link: Q9UJ83
- WB: HeLa, HEK-293T, Jurkat, NIH/3T3 and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to 2 Hydroxy phytanoyl CoA lyase/HACL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
FunctionCatalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
Tissue specificityExpressed in high levels in liver, also expressed in kidney, heart and skeletal muscle.
PathwayLipid metabolism; fatty acid metabolism.
Sequence similaritiesBelongs to the TPP enzyme family.
- Information by UniProt
- 1600020H07Rik antibody
- 2 HPCL antibody
- 2 hydroxyacyl CoA lyase 1 antibody
All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241353 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.
Lane 1: ab241353 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Chemiluminescence detection: 30 seconds.
ab241353 has not yet been referenced specifically in any publications.