Recombinant
RabMAb

Recombinant Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)

Overview

  • Product name

    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812]
    See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human 2 Hydroxy phytanoyl CoA lyase/HACL1 aa 200-400. The exact sequence is proprietary.
    Database link: Q9UJ83

  • Positive control

    • HeLa, 293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, Mouse heart, Rat brain and Rat heart lysates; 293T cells; HeLa cells.
  • General notes

     

     

     This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP 1/50.
Flow Cyt 1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
  • Tissue specificity

    Expressed in high levels in liver, also expressed in kidney, heart and skeletal muscle.
  • Pathway

    Lipid metabolism; fatty acid metabolism.
  • Sequence similarities

    Belongs to the TPP enzyme family.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1600020H07Rik antibody
    • 2 HPCL antibody
    • 2 hydroxyacyl CoA lyase 1 antibody
    • 2 hydroxyphytanoyl CoA lyase antibody
    • 2-HPCL antibody
    • 2-hydroxyacyl-CoA lyase 1 antibody
    • 2-hydroxyphytanoyl-CoA lyase antibody
    • FLJ55041 antibody
    • Hacl1 antibody
    • HACL1_HUMAN antibody
    • HPCL antibody
    • HPCL2 antibody
    • HSPC279 antibody
    • PHYH2 antibody
    • Phytanoyl CoA 2 hydroxylase 2 antibody
    • Phytanoyl CoA hydroxylase 2 antibody
    • Phytanoyl-CoA 2-hydroxylase 2 antibody
    see all

Images

  • All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Das Y  et al. Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina. Mol Cell Biochem N/A:N/A (2019). Read more (PubMed: 30604065) »
See 1 Publication for this product

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