2-NBD-Glucose, Fluorescent glucose uptake probe (ab146200)

Overview

  • Product name

    2-NBD-Glucose, Fluorescent glucose uptake probe
  • Description

    Cell-permeable fluorescent glucose uptake probe
  • Alternative names

    • 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose
    • 2-NBDG
    • NBD-Glucose
  • Biological description

    Fluorescently-labeled deoxyglucose analog, used to directly monitor glucose uptake by living cells and tissues. Also used as a topical contrast reagent for the detection of neoplasia. Uptake can be monitored by real-time confocal, high-resolution or wide-field fluorescence microscopy and flow cytometry.

  • CAS Number

    186689-07-6
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    465nm
  • Emission

    540nm
  • Molecular weight

    342.26
  • Molecular formula

    C12H14N4O8
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 50 mM and in DMSO to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    c1cc(c2c(c1N[C@@H]3[C@H]([C@@H]([C@H](OC3O)CO)O)O)non2)[N+](=O)[O-]
  • Source

    Synthetic

References

This product has been referenced in:

  • Thekkek N  et al. Pre-clinical evaluation of fluorescent deoxyglucose as a topical contrast agent for the detection of Barrett's-associated neoplasia during confocal imaging. Technol Cancer Res Treat 10:431-41 (2011). Read more (PubMed: 21895028) »
  • Langsner RJ  et al. Wide-field imaging of fluorescent deoxy-glucose in ex vivo malignant and normal breast tissue. Biomed Opt Express 2:1514-23 (2011). Read more (PubMed: 21698015) »
  • Zou C  et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods 64:207-15 (2005). Read more (PubMed: 16182371) »
  • Loaiza A  et al. Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy. J Neurosci 23:7337-42 (2003). Read more (PubMed: 12917367) »
  • Yoshioka K  et al. A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli. Biochim Biophys Acta 1289:5-9 (1996). Read more (PubMed: 8605231) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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