Overview

  • Product name

    2-NBDG Glucose Uptake Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.


    The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.


    2-NBDG protocol summary:
    - treat cells with experimental treatment in glucose-free medium
    - add 2-NBDG and incubate for 10 min
    - remove cell culture supernatant and wash cells with cell-based assay buffer
    - add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry
    - add cell-based assay buffer and analyze immediately
    2-NDG can be detected with filters designed to detect FITC (Ex/Em 485/535 nm).

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    Cell-Based Assay Apigenin 1 x 100µl
    Cell-Based Assay Buffer Tablet 1 tablet
    Cell-Based Assay NBD Glucose 1 x 500µl
    Cell-Based Propidium Iodide Solution 1 x 250µl

Images

  • Jurkat (Human T cell leukemia cell line from peripheral blood) cells were equilibrated in glucose-free medium for two hours prior to being treated with the indicated dilution of NBD-glucose for 10 minutes at 37°C. Cells were washed and data were collected on a MACSQuant cytometer. NBD-glucose fluorescence within the live population is shown.

Protocols

References

ab235976 has not yet been referenced specifically in any publications.

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