2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (ab174097)

Overview

  • Product name
    2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric)
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts
  • Assay type
    Quantitative
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mammals
  • Product overview

    Abcam’s 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool  for analyzing the glycolytic pathway and its relevance to cancer research.

  • Platform
    Microplate

Properties

Images

  • Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.

  • Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by fluorometric reading. Please not this data is example data only.

  • Measurement of 2PG level in rat liver, heart and muscle lysate (200 µg protein each). Assays were performed according to kit protocol. This is example data only.

Protocols

References

ab174097 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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