Overview

  • Product name
    20x PBS buffer with Tween 20
  • Tested applications
    Suitable for: IHC-Pmore details
  • General notes
    In order to provide this product at 20x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at room temperature.
  • Storage buffer
    pH: 7.60
    Constituents: 1% Tween, PBS
    Note: 20x concentrated.
  • Concentration information loading...
  • Research areas
  • Relevance
    PBS (Phosphate Buffered Saline) and Tween 20 are used as a wash buffer in immunolabeling techniques. Tween 20 is added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
  • Alternative names
    • PBS with Tween 20
    • Phosphate Buffered Saline and Tween 20
    • Phosphate Buffered Saline with Tween 20

Applications

Our Abpromise guarantee covers the use of ab64247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References

This product has been referenced in:
  • Celebi AR  et al. Evaluation of the 'Hedgehog' signaling pathways in squamous and basal cell carcinomas of the eyelids and conjunctiva. Oncol Lett 12:467-472 (2016). Read more (PubMed: 27347166) »
  • Ishikawa H  et al. Ischemic stroke brain sends indirect cell death signals to the heart. Stroke 44:3175-82 (2013). Read more (PubMed: 24008571) »

See all 2 Publications for this product

Publishing research using ab64247? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

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For 20x PBS buffer with Tween 20 (ab64247):   What is the mix ratio for 500ml or 1 Liter? Molarity of salt types?

ab64247 is supplied as a liquid at a 20X concentration. To prepare a 500 mL solution of 1X PBS with 0.05% Tween-20, add 25 mL of the 20X stock to 475 mL ddH2O. To prepare a 1 L solution, add 50 mL of the 20X stock to 950 mL ddH2O. The 1X solutio...

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buffer mentioned in kit:
Does it refers to PBS only or PBS + Tween 20?

Thank you for contacting us.

Thewashing / rinsing buffer to be used with this kit isPBS/Tween 20.

We do carry this buffer in our catalog in 2 different sizes(125 ml and 1000 ml):

ab64028: https://www.abcam.com/20x-PBS-Buf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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