Product name20x PBS buffer with Tween 20
Tested applicationsSuitable for: IHC-Pmore details
Phosphate Buffered Saline (PBS) with Tween 20 wash buffer for use during immunolabeling techniques such as IHC.
In order to provide this product at 20x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.
Storage instructionsStore at room temperature.
Storage bufferpH: 7.60
Constituents: PBS, 1% Tween
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RelevancePBS (Phosphate Buffered Saline) and Tween 20 are used as a wash buffer in immunolabeling techniques. Tween 20 is added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
- PBS with Tween 20
- Phosphate Buffered Saline and Tween 20
- Phosphate Buffered Saline with Tween 20
Our Abpromise guarantee covers the use of ab64247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab64247 has been referenced in 2 publications.
- Celebi AR et al. Evaluation of the 'Hedgehog' signaling pathways in squamous and basal cell carcinomas of the eyelids and conjunctiva. Oncol Lett 12:467-472 (2016). PubMed: 27347166
- Ishikawa H et al. Ischemic stroke brain sends indirect cell death signals to the heart. Stroke 44:3175-82 (2013). PubMed: 24008571