20x PBS buffer with Tween 20 (ab64247)

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Overview

  • Product name

    20x PBS buffer with Tween 20

  • Tested applications

    Suitable for: IHC-Pmore details

  • General notes

    Phosphate Buffered Saline (PBS) with Tween 20 wash buffer for use during immunolabeling techniques such as IHC.

    In order to provide this product at 20x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.

Properties

  • Form

    Liquid

  • Storage instructions

    Store at room temperature.

  • Storage buffer

    pH: 7.60
    Constituents: PBS, 1% Tween

    20x concentrated.
  • Concentration information loading...

  • Research areas

  • Relevance

    PBS (Phosphate Buffered Saline) and Tween 20 are used as a wash buffer in immunolabeling techniques. Tween 20 is added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.

  • Alternative names

    • PBS with Tween 20
    • Phosphate Buffered Saline and Tween 20
    • Phosphate Buffered Saline with Tween 20

Applications

Our Abpromise guarantee covers the use of ab64247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab64247? Please let us know so that we can cite the reference in this datasheet.

ab64247 has been referenced in 2 publications.

  • Celebi AR  et al. Evaluation of the 'Hedgehog' signaling pathways in squamous and basal cell carcinomas of the eyelids and conjunctiva. Oncol Lett 12:467-472 (2016). PubMed: 27347166
  • Ishikawa H  et al. Ischemic stroke brain sends indirect cell death signals to the heart. Stroke 44:3175-82 (2013). PubMed: 24008571

Customer reviews and Q&As

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Question

For 20x PBS buffer with Tween 20 (ab64247):   What is the mix ratio for 500ml or 1 Liter? Molarity of salt types?  

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Answer

ab64247 is supplied as a liquid at a 20X concentration. To prepare a 500 mL solution of 1X PBS with 0.05% Tween-20, add 25 mL of the 20X stock to 475 mL ddH2O. To prepare a 1 L solution, add 50 mL of the 20X stock to 950 mL ddH2O. The 1X solution pH will be approximately 7.4 but we recommend that you measure and adjust the pH if necessary. The final concentration of the salts in a 1X solution is 0.133 M sodium chloride, 8.6 mM potassium phosphate dibasic, and 1.4 mM potassium phosphate monobasic.

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Question

buffer mentioned in kit:
Does it refers to PBS only or PBS + Tween 20?

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Answer

Thank you for contacting us.

Thewashing / rinsing buffer to be used with this kit isPBS/Tween 20.

We do carry this buffer in our catalog in 2 different sizes(125 ml and 1000 ml):

ab64028: https://www.abcam.com/20x-PBS-Buffer-with-Tween-20-ab64028.html
ab64247: https://www.abcam.com/20x-PBS-buffer-with-Tween-20-ab64247.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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